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No. de sistema: 000028037

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 021218m20029999ohumrzp^^^^^^z0^^^z0eng^d
040 _ _ a| ECO
c| ECO
043 _ _ a| n-mx-yu
044 _ _ a| ohu
245 0 0 a| Genetic variation within the premembrane coding region of dengue viruses from the Yucatan Peninsula of Mexico
520 1 _ a| ingle-strand conformation polymorphism (SSCP) and sequence analyses were used to characterize genetic polymorphisms and phylogenetic relationships, respectively, among dengue (DEN) viruses isolated between 1980 and 1997 from Yucatan, Mexico and surrounding states. Amplified cDNAs from the premembrane (prM) coding region of the DEN viruses were characterized by SSCP. There were six distinct haplotypes of DEN-1 viruses, four haplotypes of DEN-2, four haplotypes of DEN-3, and eight haplotypes of DEN-4. The diversity index for DEN-3 isolates was significantly lower than that of the other serotypes, probably reflecting the recent introduction of this viral serotype into Mexico. The SSCP was a sensitive (84.5%) and specific (95.5%) technique for identifying nucleotide substitutions. Sequence analyses provided insight into the phylogenetic relationships of the DEN strains isolated in Yucatan. One DEN-2 isolate from 1996 was demonstrated to cluster with viruses of the Sri Lanka genotype, none of which have been detected before in the Americas.
650 _ 4 a| Dengue
650 _ 4 a| Variación genética
650 _ 4 a| Epidemiología
651 _ 4 a| Yucatán (Península) (México)
700 1 _ a| Díaz, Francisco J.
700 1 _ a| Farfán Ale, José A.
e| coaut.
700 1 _ a| Olson, Ken E.
e| coaut.
700 1 _ a| Loroño Pino, María Alba
e| coaut.
773 0 _
t| The American Journal of Tropical Medicine and Hygiene
g| Vol. 67, no. 1 (July 2002), p. 93-101
x| 0002-9637
856 4 1 u| http://www.ajtmh.org/content/67/1/93.full.pdf
z| Artículo electrónico
900 _ _ a| En hemeroteca, SIBE-San Cristóbal
902 _ _ a| MM
905 _ _ a| Artfrosur
905 _ _ a| Biblioelectrónica
LNG eng
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*En hemeroteca, SIBE-San Cristóbal
Genetic variation within the premembrane coding region of dengue viruses from the Yucatan Peninsula of Mexico
Díaz, Francisco J. (autor)
Farfán Ale, José A. (autor)
Olson, Ken E. (autor)
Loroño Pino, María Alba (autor)
Contenido en: The American Journal of Tropical Medicine and Hygiene. Vol. 67, no. 1 (July 2002), p. 93-101. ISSN: 0002-9637
No. de sistema: 28037
Tipo: Artículo
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Inglés

"ingle-strand conformation polymorphism (SSCP) and sequence analyses were used to characterize genetic polymorphisms and phylogenetic relationships, respectively, among dengue (DEN) viruses isolated between 1980 and 1997 from Yucatan, Mexico and surrounding states. Amplified cDNAs from the premembrane (prM) coding region of the DEN viruses were characterized by SSCP. There were six distinct haplotypes of DEN-1 viruses, four haplotypes of DEN-2, four haplotypes of DEN-3, and eight haplotypes of DEN-4. The diversity index for DEN-3 isolates was significantly lower than that of the other serotypes, probably reflecting the recent introduction of this viral serotype into Mexico. The SSCP was a sensitive (84.5%) and specific (95.5%) technique for identifying nucleotide substitutions. Sequence analyses provided insight into the phylogenetic relationships of the DEN strains isolated in Yucatan. One DEN-2 isolate from 1996 was demonstrated to cluster with viruses of the Sri Lanka genotype, none of which have been detected before in the Americas."