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No. de sistema: 000058065

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 170316m20179999xx^^r^p^^^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
043 _ _ a| n-mx-cp
044 _ _ a| xx
245 0 3 a| In vitro callogenesis induction of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae)
246 1 1 a| Inducción de callogénisis in vitro de Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae)
520 1 _ a| Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considered as threatened species by NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and the illegal collection during its flowering season. The aim of this research was to induce in vitro callogenesis from different type of explants, using phytoregulators, in order to look for a massive production technique to contribute to its conservation. We evaluated the leaf and pseudobulb pith explants growing in semi-solid medium MS adding BAP, 2, 4-D, Kin, the interaction of BAP/2, 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed the pseudobulb pith explants are more suitable for in vitro introduction in comparison to leaf explants, since they perform a lower percentage of contamination (18.8% in pith and 73.2% in leaves). Likewise, the pseudobulb pith explants increased callus formation (10.8%) in comparison to leaf explants (7.6%). Regarding the phytoregulators employed, BAP have allowed to increased callus formation (17%) compared to other phytoregulators (7-10%). This is the first report, which proposes the use of pseudobulb pith as explant for callus induction in G. skinneri.
650 _ 4 a| Guarianthe skinneri
650 _ 4 a| Orquídeas
650 _ 4 a| Formación del callo
650 _ 4 a| Cultivo in vitro
650 _ 4 a| Sustancias de crecimiento vegetal
651 _ 4 a| Tapachula (Chiapas, México)
700 1 _ a| Coutiño Cortés, Ana Gabriela
e| coaut.
700 1 _ a| Bertolini, Vincenzo
c| Dr.
e| coaut.
n| 56285906500
700 1 _ a| Iracheta Donjuan, Leobardo
e| coaut.
700 1 _ a| Ruiz Montoya, Lorena
d| 1964-
e| coaut.
700 1 _ a| Valle Mora, Javier Francisco
e| coaut.
n| 7101953229
773 0 _
t| Acta Agronomica
g| Vol. 66, no. 2 (2017), p. 254-259
x| 2323-0118
856 4 1 u| http://www.revistas.unal.edu.co/index.php/acta_agronomica/article/view/57982
z| Artículo electrónico
902 _ _ a| BG / MM
904 _ _ a| Enero 2017
905 _ _ a| Artecosur
905 _ _ a| Artfrosur
905 _ _ a| Biblioelectrónica
LNG eng
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In vitro callogenesis induction of Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae) = Inducción de callogénisis in vitro de Guarianthe skinneri (Bateman) Dressler & W.E. Higgins (Orchidaceae)
Coutiño Cortés, Ana Gabriela (autor)
Bertolini, Vincenzo (autor)
Iracheta Donjuan, Leobardo (autor)
Ruiz Montoya, Lorena, 1964- (autor)
Valle Mora, Javier Francisco (autor)
Contenido en: Acta Agronomica. Vol. 66, no. 2 (2017), p. 254-259. ISSN: 2323-0118
No. de sistema: 58065
Tipo: Artículo
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"Guarianthe skinneri (Bateman) Dressler & W.E. Higgins., is a native orchid from Mexico, considered as threatened species by NOM-ECOL-059-SEMARNAT-2010, mainly due to the disappearance of its natural habitat and the illegal collection during its flowering season. The aim of this research was to induce in vitro callogenesis from different type of explants, using phytoregulators, in order to look for a massive production technique to contribute to its conservation. We evaluated the leaf and pseudobulb pith explants growing in semi-solid medium MS adding BAP, 2, 4-D, Kin, the interaction of BAP/2, 4-D/Kin/Sad and a control without any type of plant growth regulators. Statistical analysis showed the pseudobulb pith explants are more suitable for in vitro introduction in comparison to leaf explants, since they perform a lower percentage of contamination (18.8% in pith and 73.2% in leaves). Likewise, the pseudobulb pith explants increased callus formation (10.8%) in comparison to leaf explants (7.6%). Regarding the phytoregulators employed, BAP have allowed to increased callus formation (17%) compared to other phytoregulators (7-10%). This is the first report, which proposes the use of pseudobulb pith as explant for callus induction in G. skinneri."