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No. de sistema: 000059769

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c| ECO
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245 0 0 a| Phylogeographic and population genetic analyses of Cucurbita moschata reveal divergence of two mitochondrial lineages linked to an elevational gradient
506 _ _ a| Disponible para usuarios de ECOSUR con su clave de acceso
520 1 _ a| Premise: Domestication usually involves local adaptation to environmental conditions. Cucurbita species are a promising model for studying these processes. Cucurbita moschata is the third major crop in the genus because of its economic value and because it displays high landrace diversity, but research about its genetic diversity, population structure, and phylogeography is limited. We aimed at understanding how geography and elevation shape the distribution of genetic diversity in C. moschata landraces in Mexico. Methods: We sampled fruits from 24 localities throughout Mexico. We assessed 11 nuclear microsatellite loci, one mtDNA region, and three cpDNA regions but found no variation in cpDNA. We explored genetic structure with cluster analysis, and phylogeographic relationships with haplotype network analysis. Results: Mitochondrial genetic diversity was high, and nuclear genetic differentiation among localities was intermediate compared to other domesticated Cucurbita. We found high levels of inbreeding. We recovered two mitochondrial lineages: highland (associated with the Trans-Mexican Volcanic Belt) and lowland. Nuclear microsatellites show that localities from the Yucatan Peninsula constitute a well-differentiated group. Conclusions: Mexico is an area of high diversity for C. moschata, and these landraces represent important plant genetic resources. In Mexico this species is characterized by divergence processes linked to an elevational gradient, which could be related to adaptation and may be of value for applications in agriculture. The Isthmus of Tehuantepec may be a partial barrier to gene flow. Morphological variation, agricultural management, and cultural differences may be related to this pattern of genetic structure, but further studies are needed.
533 _ _ a| Reproducción electrónica en formato PDF
538 _ _ a| Adobe Acrobat profesional 6.0 o superior
650 _ 4 a| Cucurbita moschata
650 _ 4 a| Genética de población
650 _ 4 a| Filogeografía
651 _ 4 a| México
700 1 _ a| Hernández Rosales, Helena S.
e| autora
700 1 _ a| Castellanos Morales, Gabriela
e| autora
n| 56237450200
700 1 _ a| Sánchez de la Vega, Guillermo
e| autor
700 1 _ a| Aguirre Planter, Erika
e| autora
700 1 _ a| Montes Hernández, Salvador
e| autor
700 1 _ a| Lira Saade, Rafael
e| autor
700 1 _ a| Eguiarte Fruns, Luis Enrique
e| autor
773 0 _
t| American Journal of Botany
g| Vol. 107, no. 3 (2020), p. 1–16.
x| 1537-2197
900 _ _ a| Solicítelo con su bibliotecario/a
902 _ _ a| BG / MM
904 _ _ a| Marzo 2020
905 _ _ a| Artecosur
905 _ _ a| Biblioelectrónica
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Phylogeographic and population genetic analyses of Cucurbita moschata reveal divergence of two mitochondrial lineages linked to an elevational gradient
Hernández Rosales, Helena S. (autora)
Castellanos Morales, Gabriela (autora)
Sánchez de la Vega, Guillermo (autor)
Aguirre Planter, Erika (autora)
Montes Hernández, Salvador (autor)
Lira Saade, Rafael (autor)
Eguiarte Fruns, Luis Enrique (autor)
Nota: Disponible para usuarios de ECOSUR con su clave de acceso
Contenido en: American Journal of Botany. Vol. 107, no. 3 (2020), p. 1–16.. ISSN: 1537-2197
No. de sistema: 59769
Tipo: Artículo
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"Premise: Domestication usually involves local adaptation to environmental conditions. Cucurbita species are a promising model for studying these processes. Cucurbita moschata is the third major crop in the genus because of its economic value and because it displays high landrace diversity, but research about its genetic diversity, population structure, and phylogeography is limited. We aimed at understanding how geography and elevation shape the distribution of genetic diversity in C. moschata landraces in Mexico. Methods: We sampled fruits from 24 localities throughout Mexico. We assessed 11 nuclear microsatellite loci, one mtDNA region, and three cpDNA regions but found no variation in cpDNA. We explored genetic structure with cluster analysis, and phylogeographic relationships with haplotype network analysis. Results: Mitochondrial genetic diversity was high, and nuclear genetic differentiation among localities was intermediate compared to other domesticated Cucurbita. We found high levels of inbreeding. We recovered two mitochondrial lineages: highland (associated with the Trans-Mexican Volcanic Belt) and lowland. Nuclear microsatellites show that localities from the Yucatan Peninsula constitute a well-differentiated group. Conclusions: Mexico is an area of high diversity for C. moschata, and these landraces represent important plant genetic resources. In Mexico this species is characterized by divergence processes linked to an elevational gradient, which could be related to adaptation and may be of value for applications in agriculture. The Isthmus of Tehuantepec may be a partial barrier to gene flow. Morphological variation, agricultural management, and cultural differences may be related to this pattern of genetic structure, but further studies are needed."


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