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73 resultados encontrados para: AUTOR: Sánchez Guillén, Daniel
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1.
Artículo
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(S)-2-Heptanol, the alarm pheromone of the stingless bee Melipona solani (Hymenoptera, Meliponini)
Alavez Rosas, David (autor) ; Sánchez Guillén, Daniel (autor) ; Malo Rivera, Edi Álvaro (autor) ; Cruz López, Leopoldo Caridad (autor) ;
Disponible en línea
Contenido en: Apidologie Vol. 50, no. 3 (July 2019), p. 277-287 ISSN: 1297-9678
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Resumen en: Inglés |
Resumen en inglés

Timely defence response is critical in any highly social bee species. Chemical signalling is closely linked to this behaviour, and several pheromones have been discovered. In this work, we identified the pheromone ofthe stingless bee Melipona solani and evaluated the electrophysiological and behavioural response of the bee to the identified compound. We determined that the mandibular glands serve as the reservoir of the alarm pheromone. Because enantiomeric recognition has been scarcely examined, we performed bioassays and electroantennographic (EAG) studies to determine the role of chirality in pheromone recognition. We found that (S)-2-heptanol was the active compound involved in the alarm response of this species. Although we did not find (R)-2-heptanol in the mandibular gland extracts, bees responded to it in a similar way to the (S)-isomer and to a racemic mixture. The behavioural response of M. solani was flight, different from other Melipona species behaviour. We discuss these findings in an evolutionary and ecological framework.


2.
Tesis - Maestría
Degradación de plaguicidas por la microbiota intestinal de abejas silvestres del Soconusco / Augusto Campollo Ovalle
Campollo Ovalle, Augusto (autor) ; Sánchez Guillén, Daniel (director) ; Guillén Navarro, Griselda Karina (asesora) ; Gómez Ruiz, Jaime (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2019
Clasificación: TE/638.1097275 / C3
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013844 (Disponible)
Disponibles para prestamo: 1
Resumen en español

Diversas especies de insectos han desarrollado resistencia o tolerancia a insecticidas. Aunque la presencia de mutaciones en genes relacionados con la degradación o insensibilidad a insecticidas se encuentra bien demostrada se han descubierto microorganismos endosimbiontes capaces de neutralizar la toxicidad de dichos compuestos, proporcionando resistencia a sus hospederos. En Apis mellifera, se han reportado microorganismos benéficos que aparentemente están involucrados en la degradación de pesticidas. El objetivo del trabajo fue determinar si la microbiota intestinal de A. mellifera y Scaptotrigona mexicana presentes en el Soconusco, Chiapas, es capaz de degradar al insecticida malatión debido al uso constante en campo; además se consideró al plaguicida spinetoram como un testigo de reciente utlización en campo al que no han desarrollado tolerancia. Se llevó a cabo el siguiente procedimiento experimental: 1) se realizaron colectas de abejas en apiarios y meliponarios en zonas agrícolas del Soconusco, expuestos continuamente a insecticidas; 2) se determinó la presencia de malatión en cuerpos de abejas mediante extracciones por el método de QuEChERS y se analizaron mediante cromatografía de gases; 3) se calculó la DL50 y CL50 de ambas especies a ambos insecticidas; 4) se disectaron los tractos digestivos de abejas, para evaluar los consorcios bacterianos en medio M9, enriquecido con ambos plaguicidas a diversas concentraciones. No se encontraron residuos de malatión en los especímenes analizados. La DL50 y la CL50 obtenida fue parecida a los obtenidos en estudios previos, indicando que las abejas no habían desarrollado tolerancia al malatión ni al spinetoram. No se obtuvieron consorcios bacterianos del tracto digestivo de abejas capaces de degradar malatión ni spinetoram

Índice

Dedicatoria
Agradecimientos
Resumen
Introducción
Artículo: Microbial gut consortia from two highly social bee species is not involved on the degradation of the pesticides malathion and spinetoram
Conclusiones
Literatura citada


3.
Tesis - Doctorado
Efecto de agroquímicos utilizados en cultivos tropicales sobre polinizadores / Jovani Ruiz Toledo
Ruiz Toledo, Jovani (autor) ; Sánchez Guillén, Daniel (director) ; Penilla Navarro, Rosa Patricia (asesora) ; Vandame, Rémy (asesor) ; Gómez Ruiz, Jaime (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2019
Clasificación: TE/638.16097275 / R8
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013845 (Disponible)
Disponibles para prestamo: 1
Resumen en español

El objetivo de este estudio fue determinar la variación espacial y estacional de plaguicidas en miel y polen, en la actividad de enzimas de detoxificación y su efecto sobre la diversidad de abejas silvestres en un agropaisaje de la ciudad de Tapachula, Chiapas de junio 2015 a mayo 2016. Se seleccionaron cuatro sitios, en donde se realizaron colectas mensuales de abejas silvestres. Cada mes también se tomaron muestras de polen, miel y abejas forrajeras de colonias de Apis mellifera y Scaptotrigona mexicana cercanas a las zonas agrícolas estudiadas. Se colectaron 2,115 especímenes silvestres; las especies más abundantes fueron Augochlora (Oxystoglossella) aurifera (12.8%), Trigona fuscipennis (9.2%), y T. fulviventris (8.6%). Se observó una alta diversidad durante la temporada de lluvias y en los sitios con cultivos de soya. Encontramos que el 88.44% y el 93.33% de las muestras de miel, y el 22.22% y el 100% de las muestras de polen de S. mexicana y A. mellifera, respectivamente, resultaron positivas al menos a un organoclorado. Los plaguicidas más abundantes fueron Heptaclor (44% de las muestras), γ-HCH (36%), DDT (19%), Endrin (18%) y DDE (11%). A pesar del corto rango de alimentación de S. mexicana, el número de plaguicidas cuantificados en las muestras de miel fue similar al de A. mellifera. En el análisis de las enzimas Glutatión-S-transferasa (GST), Citocromo P450, acetilcolinesterasa (AChE), α esterasa, β esterasa y ρNPA en ambas especies de abejas, se encontraron diferencias significativas entre especies, siendo S. mexicana quien mostró mayores expresiones enzimáticas. No se observaron diferencias significativas en la expresión de enzimas a lo largo del tiempo o entre paisajes para cada especie.

Nuestros resultados sugieren que las condiciones actuales no parecen afectar a las poblaciones de polinizadores debido a que han desarrollado tolerancia a las concentraciones de plaguicidas en campo, ya que no se observaron altas tasas de mortalidad en las colonias a pesar de la fuerte presencia de plaguicidas organoclorados en el ambiente.

Índice

Resumen y Palabras clave
Capítulo I. Introducción
Capítulo II. Objetivos e Hipótesis
Capítulo III. Seasonal abundance and diversity of native bees in herbicide-resistant soybean field margins and forest relicts in a patchy agricultural landscape in Southern Mexico
Capítulo IV. Organochlorine Pesticides in Honey and Pollen Samples from Managed Colonies of the Honey Bee Apis mellifera Linnaeus and the Stingless Bee Scaptotrigona mexicana Guérin from Southern, Mexico
Capítulo V. Variación temporal de biomarcadores enzimáticos de detoxificación en las abejas Apis mellifera y Scaptotrigona mexicana
Capítulo VI. Discusión y Conclusión


4.
- Artículo con arbitraje
Seasonal abundance and diversity of native bees in a patchy agricultural landscape in Southern Mexico
Ruiz Toledo, Jovani (autor) ; Vandame, Rémy (autor) ; Penilla Navarro, Rosa Patricia (autora) ; Gómez Ruiz, Jaime (autor) ; Sánchez Guillén, Daniel (autor) ;
Disponible en línea
Contenido en: Agriculture, Ecosystems & Environment Vol. 292, no. e106807 (2019), p. 1-8 ISBN:0167-8809
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Resumen en: Inglés |
Resumen en inglés

Diversity and abundance of native bees was measured in four sites of an agricultural landscape dominated by soybean crops in Southern Mexico. Bees were sampled from June 2014 to May 2015 using entomological nets along five 500 m long transects in each site. Two sites (S1 and S2) were fully anthropized (100% of urban and agricultural land use) and the two other sites (R1 and R2) were on average less anthropized with 27.5% of forest relicts. Overall, we collected 2115 specimens; the most abundant species were Augochlora (Oxystoglossella) aurifera (12.8%), Trigona fuscipennis (9.2%), and T. fulviventris (8.6%). The highest diversity was observed in June and July (rainy season), in sites S2 and S1. Site S2 had the greatest diversity and abundance (56 species, 885 individuals), followed by S1 (41 species, 577 individuals), R2 (38 species, 289 individuals) and R1 (25 species, 117 individuals). However, rarefaction analysis showed no significant difference among four sites. Compared to studies carried out in protected natural areas with similar climatic conditions, our results suggest that small-scale agriculture in southern Mexico apparently does not affect detrimentally pollinator populations as expected. Thus, this study suggest that the presence of preserved patches contributes to the richness and abundance of bees, due to the maintenance of wild flowers and ruderal plants in patches next to the crop fields, providing a continuous source of pollen, nectar and shelters. Nonetheless, further studies are necessary to examine the connections between the different elements in the agricultural landscape and to understand in depth the response of the pollinator communities.


5.
- Artículo con arbitraje
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Associative learning of non-nestmate odor marks between colonies of the stingless bee Scaptotrigona mexicana Guérin (Apidae, Meliponini) during foraging
Villanueva Gutiérrez, Rogel (autor) ; Solórzano Gordillo, Erik de Jesús (autor) ; Rojas, Julio C. (autor) ; Cruz López, Leopoldo Caridad (autor) ; Sánchez Guillén, Daniel (autor) ;
Disponible en línea
Contenido en: Insectes Sociaux Vol. 65, no. 3 (August 2018), p. 393-00 ISSN: 0020-1812
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Resumen en: Inglés |
Resumen en inglés

Stingless bees use chemical signals to communicate nestmates the location of rich food sources. Such information may be intercepted by conspecifics from other colonies. In this study, we investigated if chemical information from non-nestmates can be used to orient foragers of the stingless bee Scaptotrigona mexicana to food sources. In the first experiment, foragers were exposed to feeders that were differentially odor-marked by nestmates and non-nestmates, and their preferences for both types of feeders were recorded. In a second experiment, we marked different feeders with mandibular or labial gland extracts of nestmates and non-nestmates. Results from the first experiment indicate that foragers were able to associate odor marks from non-nestmates with rich food sources. In the second experiment, we observed that foragers did not differentiate between the gland extracts of nestmates and those from non-nestmates. We discuss these findings within a behavioral and ecological framework.


6.
- Artículo con arbitraje
PDF PDF
Resumen en: Inglés |
Resumen en inglés

We studied the volatile composition and sexual morphs of Nephelium lappaceum flowers from two orchards, and investigated the choice behavior of the honey bee, Apis mellifera toward the floral extracts from both locations. Our results showed significant differences in chemical composition and sexual morphs; only the hermaphrodite flowers from the Herradero orchard produced limonene and α-pinene and had longer peduncle and sepal than flowers from the Metapa orchard; on the other hand, the hermaphrodite flowers from the Metapa orchard had longer gynoecium. In the behavioral experiment the extracts from the Herradero orchard seemed to give A. mellifera foragers better cues for orientation to food sources, perhaps due to the presence of limonene and α-pinene, which are absent in the samples from Metapa. Such differences in both orchards could affect pollinator attraction and ultimately seed set and productivity.


7.
- Artículo con arbitraje
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Effect of GF-120 (Spinosad) aerial sprays on colonies of the stingless bee Scaptotrigona mexicana (Hymenoptera: Apidae) and the honey bee (Hymenoptera: Apidae)
Gómez Escobar, Enoc ; Liedo Fernández, Pablo (coaut.) ; Montoya Gerardo, Pablo Jesús (coaut.) ; Méndez Villareal, Agustín (coaut.) ; Guzmán Díaz, Miguel Ángel (coaut.) ; Vandame, Rémy (coaut.) ; Sánchez Guillén, Daniel (coaut.) ;
Contenido en: Journal of Economic Entomology Vol. 111, no. 4 (August 2018), p. 1711-1715 ISSN: 0022-0493
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

Despite their relevant contribution to the conservation of tropical ecosystems and crop productivity through pollination, the stingless bees (Apidae: Meliponini) can be considered a group of neglected species in the assessment of pesticides upon nontarget organisms. In this article, we evaluated the effect of aerial sprays of the spinosad-based fruit fly toxic bait GF-120 upon colonies of the stingless bee Scaptotrigona mexicana Guérin (Hymenoptera: Apidae), an economically important and abundant species in some landscapes of Mexico, located in mango orchards. Colonies of the honey bee Apis mellifera L. (Hymenoptera: Apidae) were used for comparison. Eight colonies (four of A. mellifera and four of S. mexicana ) were moved into each of two mango orchards, one was used as a control, with no insecticide application, and other received five weekly aerial sprays of GF-120. Foraging activity and strength of colonies of both species were measured nine times over the fruiting season, previous, during and after insecticide application. We did not find a significant difference in foraging activity and strength between exposed and control colonies of A. mellifera during the observation period. However, colonies of S. mexicana seemed to be affected by the exposure, as revealed by a reduction in colony strength. However, 1 yr later, with no insecticide applications, the colonies of both species were evaluated and found to be in good conditions. Our results showed that weekly aerial sprays of GF-120 are unlikely to generate acute poisoning in both species, even if in acute toxicity tests this product has been found to be highly active.


8.
- Artículo con arbitraje
Effect of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) on food consumption and mortality in the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae)
Toledo Hernández, Ricardo Alberto ; Toledo, Jorge (coaut.) ; Sánchez Guillén, Daniel (coaut.) ;
Contenido en: International Journal of Tropical Insect Science Vol. 38, no. 3 (September 2018), p. 254-260 ISSN: 1742-7592
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Resumen en: Inglés |
Resumen en inglés

Anastrepha ludens (Loew) (Diptera: Tephritidae) is an important pest of mango and citrus in Mexico. Here, we report the effect of the entomopathogenic fungus Metarhizium anisopliae (Metsch.) Sorokin strain MAAP1 on mortality and food consumption in A. ludens adults. Experiments were carried out under laboratory conditions at 26 ± 2 °C, 60 ± 5% RH and 12:12 photoperiod. To assess pathogenicity and sublethal effects, we sprayed flies with 1 × 109 conidia/ml and 1 × 106 conidia/ml solutions, respectively. Food consumption was measured by providing known amounts of food through capillary tubes to caged flies. We found that M. anisopliae was highly virulent to A. ludens, causing 92.3 ± 0.62% mortality at day 13 post-exposure. Interestingly, food consumption reduced by 17% in flies exposed to the fungus. These results support the implementation of M. anisopliae within current strategies used for the integrated pest management of A. ludens.


9.
- Artículo con arbitraje
*En hemeroteca, SIBE-San Cristóbal
Genetic analysis of wild drone congregations of the stingless bee Scaptotrigona mexicana (Hymenoptera: Apidae) reveals a high number of colonies in a natural protected area in Southern Mexico
Sánchez Guillén, Daniel ; Vandame, Rémy (coaut.) ; Kraus, Frank Bernhard (coaut.) ;
Contenido en: Revista Mexicana de Biodiversidad Vol. 89, no. 1 (marzo 2018), p. 226-231 ISSN: 1870-3453
Nota: En hemeroteca, SIBE-San Cristóbal
PDF
Resumen en: Español | Inglés |
Resumen en español

En el presente trabajo se analizaron genéticamente congregaciones de zánganos de la abeja sin aguijón Scaptotrigona mexicana Guérin en 5 loci tipo microsatélite para estimar el número de colonias de esta especie en la Reserva de la Biosfera La Sepultura en Chiapas, México. En total se analizaron 216 zánganos provenientes de 3 congregaciones. Se encontró que las 3 tuvieron suficientes diferencias genéticas para ser consideradas de diferentes poblaciones El número promedio (± DE) de colonias por congregación fue de 19.8 ± 0.63, 17.6 ± 0.70 y 12; un número similar se reportó en áreas donde hay meliponicultura. Se discuten las implicaciones de estos hallazgos desde una perspectiva de conservación en la que la meliponicultura podría jugar un papel muy importante.

Resumen en inglés

In this study, drone congregations of the stingless bee Scaptotrigona mexicana Guérin were genetically analyzed at 5 microsatellite loci to estimate the number of colonies of this species in the La Sepultura Biosphere Reserve, in Chiapas, Mexico. A total of 216 drones from 3 congregations were analyzed. We found that the 3 congregations had enough genetic differences to be considered as coming from different populations. The average number (± SD) of colonies per congregation was 19.8 ± 0.63, 17.6 ± 0.70 and 12. A similar result was reported for areas where stingless beekeeping occurs. We discuss the implications of our findings in a conservation framework in which stingless beekeeping might play a significant role.


10.
- Artículo sin arbitraje
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Geographic variation in pheromone component ratio and antennal responses, but not in attraction, to sex pheromones among Fall armyworm populations infesting corn in Mexico
Cruz Esteban, Samuel ; Rojas, Julio C. (coaut.) ; Sánchez Guillén, Daniel (coaut.) ; Cruz López, Leopoldo Caridad (coaut.) ; Malo Rivera, Edi Álvaro (coaut.) ;
Contenido en: Journal of Pest Science Vol. 91, no. 3 (June 2018), p. 973–983 ISSN: 1612-4758
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Resumen en: Inglés |
Resumen en inglés

The fall armyworm, Spodoptera frugiperda (Lepidoptera: Noctuidae), is one of the most important pests of corn in Latin America. This insect presents two strains with behavioural and genetic differences. In Mexico, both strains are present, and at least two different FAW populations have been reported within the corn-strain. The objective of this study was to gather evidence of whether pheromonal communication varies among different S. frugiperda populations infesting corn in Mexico. First, we investigated any qualitative or quantitative difference in the composition of sex pheromones among populations; second, we studied whether male antennal responses to pheromone components vary among populations; and, finally, we investigated whether males from a region can discriminate between a synthetic pheromone blend characteristic of their region and blends formulated with the ratio of pheromone compounds emitted by females from other populations. Sex pheromone components were sampled by solid-phase microextraction and identified by gas chromatography coupled to mass spectrometry. Females from all populations consistently released three compounds: (Z)-9-tetradecenyl acetate (Z9-14:OAc), (Z)-11-hexadecenyl acetate (Z11-16:OAc) and (Z)-7-dodecenyl acetate (Z7-12:OAc). Our results showed that Z9-14:OAc and Z7-12:OAc evoked the highest and most variable antennal responses among the populations studied compared to Z11-16:OAc. However, males did not discriminate between local pheromone blends and those formulated from other populations in a field test. These results show that although there is geographic variation in the ratio of pheromone components and in the peripheral reception of them, males were not differentially attracted to different pheromone blends in the field.