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44 resultados encontrados para: TEMA: Agaricus
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1.
Tesis - Doctorado
Evaluación de la degradación de fármacos por una lacasa de Pleurotus djamor inmovilizada sobre Nylo-6 / Rosbi Cruz Ornelas
Cruz Ornelas, Rosbi (autora) ; Calixto Romo, María de los Ángeles (directora) ; Amaya Delgado, Lorena (asesora) ; Guillén Navarro, Griselda Karina (asesora) ; Sánchez, José E. (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2019
Clasificación: TE/579.6163 / C7
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013859 (Disponible)
Disponibles para prestamo: 1
Resumen en español

Debido a su alta producción y consumo, los compuestos farmacéuticamente activos se encuentran en los cuerpos de agua formando mezclas complejas que pueden ejercer efectos tóxicos sobre algunas poblaciones de los organismos acuáticos. Esta contaminación ambiental ha evidenciado la necesidad de desarrollar diferentes técnicas para la eliminación de dichos fármacos. El objetivo de esta investigación fue evaluar la capacidad del hongo Pleurotus djamor (ECS-0123) en la degradación de antiflamatorios no esteroideos (AINEs) tales como diclofenaco, naproxeno y ketoprofeno. Primeramente, se realizaron cinéticas de degradación utilizando extractos crudos enzimáticos en el cual se evaluaron las actividades enzimáticas de lacasas, manganeso peroxidasas y lignino peroxidasas. Posteriormente se produjo, purificó e inmovilizó la enzima lacasa covalententemente sobre microesferas de Nylon-6. Se efectuó la caracterización bioquímica de las lacasas libres e inmovilizadas. Finalmente se evaluó la capacidad del biocatalizador sobre la mezcla de AINEs seleccionados. Se alcanzó la degradación de 90 % de diclofenaco en las primeras 6 h y el 80 % de degradación del ketoprofeno y naproxeno a las 48 h. Por otro lado, se monitoreó la presencia de las tres enzimas ligninoliticas (lacasas, manganeso peroxidasa y lignino peroxidasa) en P. djamor, las cuales incrementaron su actividad catalítica durante la degradación de mezcla de fármacos. Se lograron extractos de alta pureza de lacasa con un peso molecular de 41 kDa, con una mayor actividad catalítica a pH de 2.6 y una temperatura de 60 °C cuando se encontraba la enzima libre, mientras que inmovilizada presentó pH óptimo de 2 y 4.5, con temperatura óptima de 30 °C en los dos pH. Por último, se evaluó la capacidad del biocatalizador sobre la degradación de la mezcla de AINEs y se alcanzó una degradación mayor de 50 % durante las primeras 24 h.

Índice

Resumen
Capítulo I.- Introducción
Capítulo II.- Biodegradation of NSAIDs and their effect on the activity of ligninolytic enzymes from Pleurotus djamor
Capítulo III.- Degradación de Antiinflamatorios no esteroideos mediante lacasas de Pleurotus djamor inmovilizadas sobre microesferas de Nylon-6
Capitulo IV.- Conclusiones
Literatura citada


2.
Artículo
Biodegradation of NSAIDs and their effect on the activity of ligninolytic enzymes from Pleurotus djamor
Cruz Ornelas, Rosbi ; Sánchez, José E. (coaut.) ; Amaya Delgado, Lorena (coaut.) ; Guillén Navarro, Griselda Karina (coaut.) ; Calixto Romo, Angeles (coaut.) ;
Contenido en: 3 Biotech Vol. 9, no 10, 373 (October 2019), p. 1-8 ISSN: 2190-5738
PDF
Resumen en: Inglés |
Resumen en inglés

In this work, the white-rot fungus Pleurotus djamor was used for the first time to determine the degradation kinetics of the nonsteroidal anti-inflammatory drugs diclofenac, naproxen and, ketoprofen, either individually or in mixtures, in submerged cultures. Removal of 93% individual diclofenac and 99% diclofenac in mixtures with naproxen and ketoprofen at 6 h of incubation with the fungus was achieved. The elimination levels of naproxen and ketoprofen individually were 90% and 87%, respectively, after 48 h of incubation. However, the removal levels of these compounds in mixtures were 85% and 83%, respectively. On the other hand, during the degradation kinetics analysis, the enzymatic activities of laccases, manganese peroxidases, and lignin peroxidases were evaluated, yielding values of 3700, 270 and 31 U/L, respectively. Additionally, it was demonstrated that during degradation of diclofenac or the three drugs mixed in the submerged cultures, the enzymatic activity of extracellular laccases expressed by P. djamor increased by 200% and 300%, respectively. The activity of manganese peroxides increased by 126% with diclofenac and 138% when the mixture of drugs was added to the cultures. On the other hand, lignin peroxidase only increased activity by 123% with the drug mixture.


3.
- Capítulo de libro con arbitraje
Enhanced production of the medicinal mushroom Agaricus subrufescens peck
Cunha Zied, Diego ; Pardo Giménez, Arturo (coaut.) ;
Contenido en: Updates on tropical mushrooms. Basic and applied research San Cristóbal de Las Casas, Chiapas, México: El Colegio de la Frontera Sur, 2018 página 59-70 ISBN:978-607-8429-60-8
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Resumen en: Inglés |
Resumen en inglés

Agaricus subrufescens Peck [A. blazei (Murrill) ss. Heinemann] has been studied by researchers in several countries (Brazil, USA, China, Taiwan, etc.) primarily because of its medicinal and pharmacological properties. Cell walls of the fungus contain polysaccharides called β-glucans that have structural and other functions. Several studies highlight the importance of A. subrufescens medicinal properties. It was traditionally used to treat many common diseases like atherosclerosis, hepatitis, hyperlipidemia, diabetes, dermatitis and cancer. Beneficial properties of A. subrufescens include tumor growth reduction, immune modulatory activities, immune stimulatory effects, antimicrobial and antiviral activities and anti-allergy effects. In this work, various aspects of production of A. subrufescens are presented. Production of this edible medicinal mushroom has generated a notable interest worldwide in the last few years, becoming increasingly popular and as a result has expanded into other countries (Spain, France, South Korea, etc.). This is mainly due to its high international market price that is related not only to the remarkable medicinal properties contributed by high content of bioactive compounds but also to the culinary value added by its slightly nutty pleasant aroma.


4.
- Capítulo de libro con arbitraje
Tropical species of agaricus
Callac, Philippe ; Chen, Jie (coaut.) ;
Contenido en: Updates on tropical mushrooms. Basic and applied research San Cristóbal de Las Casas, Chiapas, México: El Colegio de la Frontera Sur, 2018 página 25-38 ISBN:978-607-8429-60-8
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Resumen en: Inglés |
Resumen en inglés

Agaricus is a large genus of mushrooms including species of nutritional and medicinal interest. With research programs developed over the last decade, a larger diversity of Agaricus came to light. The present review focuses on tropical species of Agaricus and their classification, including a discussion of the definition of tropical species, a brief history of the taxonomic system of classification since 2000, an updated presentation of the revised system including its recent emendation, and the distribution of tropical species in the subgenera and sections of the current system. Tropical species of interest and methods to identify tropical species of Agaricus are also introduced. The new classification system should facilitate studies of tropical Agaricus; however, more investigations in unexplored areas are necessary to complete the classification system and to clarify the evolutionary history of the genus in which climate and geography seem to have been the main factors of diversificat ion.


5.
- Libro con arbitraje
Updates on tropical mushrooms. Basic and applied research / José E. Sánchez, Gerardo Mata and Daniel J. Royse, editors
Sánchez, José E. (ed.) ; Mata, Gerardo (coed.) ; Royse, Daniel Joseph (coed.) ;
San Cristóbal de Las Casas, Chiapas, México : El Colegio de la Frontera Sur , 2018
Clasificación: EE/635.8 / U6
Bibliotecas: Campeche , San Cristóbal , Tapachula
Cerrar
SIBE Campeche
ECO040007032 (Disponible)
Disponibles para prestamo: 1
Cerrar
SIBE San Cristóbal
ECO010019577 (Disponible)
Disponibles para prestamo: 1
Cerrar
SIBE Tapachula
ECO020013831 (Disponible)
Disponibles para prestamo: 1
PDF
Índice

Prologue
Introduction
1 Importance and Potential of Tropical Mushrooms
Biodiversity and Taxonomy
2 tropical species of Agaricus
3 an overview of tropical and subtropical species of Agaricus in México
Cultivation
4 development of the straw mushroom sector in China
5 Enhanced Production of the Medicinal Mushroom Agaricus Subrufescens Peck
6 Artificial Cultivation of the Medicinal Mushroom Sparassis latifolia
7 Effect of Soak Time After Harvest on Nutritional Quality of Tremella Fuciformis Dry Product
8 Fruit Body Production of Schizophyllum Commune
9 Basidiome production of guatemalan strains of Lepista nuda
10 Shiitake cultivation on straw: an alternative for Subtropical regions
Biotechnological Applications
11 Auricularia spp.: edible mushroom with broad biotechnological properties
12 Antioxidant Activity and Chemical Composition of Grifola Frondosa
13 Immunomodulating and antitumor properties of Pleurotus sp. in Cuba
14 Cultivation Biotechnology for Volvariella spp. in Mexico: Advances, Challenges and Perspectives
15 A Pleurotus spp. hydroalcoholic fraction possess a potent in vitro ovicidal activity against the sheep parasitic nematode Haemonchus contortus
Perspectives
Appendix
1 Mushrooms mentioned in this book
2 Tropical species of Agaricus


6.
- Artículo con arbitraje
Agaricus bisporus production on substrates pasteurized by self-heating
Colmenares Cruz, Stephania (autora) ; Sánchez, José E. (autor) ; Valle Mora, Javier Francisco (autor) ;
Contenido en: AMB Express Vol. 7, no. 135 (June 2017), p. 1-9 ISSN: 2191-0855
PDF
Resumen en: Inglés |
Resumen en inglés

The aim of this work was to determine if the self-heating pasteurization procedure is technically applicable to the cultivation of Agaricus bisporus. Firstly the substrates alone (corncob, Pangola grass and a mixture of both ingredients with wood shavings) were tested. Two supplementation trials were then undertaken using soybean, wheat bran, sheep manure, sesame seed, black bean and chia. Highest production values (BE = 176.3% and Y = 26.6 kg/m²) were obtained using a 9% supplement, with a formula consisting of 25% each of soybean, black bean, wheat bran and chia, added at spawning and at casing. These results were comparable to those obtained with the Phase II compost traditionally used for A. bisporus cultivation.


7.
- Artículo con arbitraje
*Solicítelo con su bibliotecario/a
Self-Heating pasteurization of substrates for culinary-medicinal mushrooms cultivation in Mexico
Morales, Viviana (autora) ; Sánchez, José E. (autor) ;
Disponible en línea
Contenido en: International Journal of Medicinal Mushrooms Vol. 19, no. 5 (2017), p. 477-484 ISSN: 1521-9437
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

The aim of this study was to evaluate a self-heating pasteurization technique in preparing substrates for mushroom production. Seven species were used: Agrocybe aegerita, Auricularia fuscosuccinea, Pleurotus djamor, P. eryngii, P. ostreatus, Lentinula edodes, and Ganoderma lucidum. They were cultivated on grass, corncob, wood shavings, and a mixture thereof. The self-heating technique allowed for pasteurization of 3 of the substrates (grass, corncob, and the mixture). The preheating chamber comprised a drawer placed under the pasteurization crate. With this chamber, it was possible to increase inlet air temperatures by 4−5°C. The evaluated mushroom species responded in different ways to the pasteurization process. P. ostreatus (control) and P. djamor produced basidiomes when cultivated in all pasteurization substrates. A. aegerita and P. eryngii fruited only on corncob and the mixture, whereas A. fuscosuccinea fruited only on the pasteurized corncob. G. lucidum and L. edodes did not fructify on the pasteurized substrates.


8.
- Artículo con arbitraje
Box Wilson factorial design methodology for the optimization of laccase production by Pleurotus djamor in liquid Culture
Nieto López, Consuelo ; Márquez Rocha, Facundo Joaquín (coaut.) ; Sánchez, José E. (coaut.) ;
Contenido en: Current Research in Microbiology and Biotechnology Vol. 4, no. 2 (March-April 2016), p. 835-841 ISSN: 2320-2246
PDF
Resumen en: Inglés |
Resumen en inglés

A surface response design methodology was used to optimize laccase production by Pleurotus djamor. Among six strains ECS-0184 was selected to apply the mathematical approach. ECS-0184 showed a specific growth rate (μ) of 0.87 days-¹ and a laccase yield (YL/X) of 372 UA g-¹ biomass. Predictable optimal conditions obtained were pH 5–6, temperature range 25–28 °C and 3–5 days of cultivation to obtain best laccase yield, 1175 UA g-¹ biomass. Critical factors for laccase production are temperature and day of harvest according with experimental design. The laccase produced by Pleurotus djamor has a molecular weight of 44 kDa; its activity was detected in PAGE gels with ABTS and pyrogallol as substrates.


9.
Artículo
*En hemeroteca, SIBE-San Cristóbal
Broca y roya del café. Viejos problemas, nuevos enfoques
Barrera, Juan F. ;
Contenido en: Ciencia y Desarrollo Vol. 42, no. 284 (julio-agosto 2016), p. 32-37 ISSN: 0185-0008
Bibliotecas: San Cristóbal
Cerrar
SIBE San Cristóbal
57940-10 (Disponible)
Disponibles para prestamo: 1
Nota: En hemeroteca, SIBE-San Cristóbal
PDF

10.
Capítulo de libro - Memoria en extenso sin arbitraje
*Solicítelo con su bibliotecario/a
Biotechnological potential of ten Pleurotus djamor strains
Moreno Ruiz, Lilia ; Andrade Gallegos, René Humberto (coaut.) ; Sánchez, José E. (coaut.) ;
Contenido en: Proceedings of the 8th international conference on mushroom biology and mushroom products New Delhi, India : Mushroom Society of India : World Society for Mushroom Biology and Mushroom Products, 2014 Vol. I, p. 262-270
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

With the aim of knowing some capabilities of biotechnological interest of the edible mushroom Pleurotus djamor, ten strains of this species were characterized. Besides mycelial growth and sporophore production, laccase (on ABTS), Mnperoxidase (on dimethoxyphenol), aryl alcohol oxidase (on veratryl alcohol) and phenol oxidase activities (on catechol) were determined. Also the antioxidant capacity was determined. Reported radial extension rate varied between 1.12 mm/ d (P. djamor ECS-0150) and 3.9 mm/d (P. djamor ECS-0143). Biological efficiency, production rate, performance in two harvests varied between 36.4 and 71.4%, 1.14 and 2.1% and 0.033 and 0.069, respectively. All the strains tested have at a certain degree, laccase, Mn-Peroxidase and phenol oxidase, except for aryl alcohol oxidase. The strain ECS- 0123 showed important ligninolytic activity, good antioxidant capacity, good growth and an average capacity to produce sporocarps (mushrooms).