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Biosintesis y bioconversión de metabolitos secundarios por células cultivadas in vitro / Manuel L. Robert; Jorge Reyes; Víctor Manuel Loyola
Robert, Manuel L. ; Reyes, Jorge ; Loyola, Víctor Manuel ;
s. l. : s. n. , s. f
Clasificación: F/581.192072 / R6
Bibliotecas: San Cristóbal
SIBE San Cristóbal
SAF001824 (Disponible)
Disponibles para prestamo: 1

Molecular population genetics / Matthew W. Hahn
Hahn, Matthew W. ;
New York : Oxford University Press, Sinauer Associates , 2019
Clasificación: 575.15 / H38
Bibliotecas: Chetumal
SIBE Chetumal
ECO030008750 (Disponible)
Disponibles para prestamo: 1
Resumen en: Inglés |
Resumen en inglés

Molecular Population Genetics is a general text covering one of the most active and exciting areas in biology. Combining advances in molecular biology and genomics with mathematical and empirical findings from population genetics, work in molecular population genetics has uncovered the extraordinary history of natural selection and demographic shifts in many organisms, including humans. While basic descriptions of the methods and tools of this field can be found in disparate places, no previous book has brought them together in a single volume. Rather than cobble together pieces from books, reviews, and primary research articles, Molecular Population Genetics presents a coherent user's guide to the field. Intended as a text for upper-level undergraduate and graduate students, the book will also be useful as a detailed reference for active professionals.

Resumen en: Inglés |
Resumen en inglés

Since its founding, the Monterey Bay Aquarium Research Institute (MBARI) has pioneered unique capabilities for accessing the deep ocean and its inhabitants through focused peer relationships between scientists and engineers. This focus has enabled breakthroughs in our understanding of life in the sea, leading to fundamental advances in describing the biology and the ecology of open-ocean and deep-sea animals. David Packard’s founding principle was the application of technological advances to studying the deep ocean, in part because he recognized the critical importance of this habitat in a global context. Among other fields, MBARI’s science has benefited from applying novel methodologies in molecular biology and genetics, imaging systems, and in situ observations. These technologies have allowed MBARI’s bioluminescence and biodiversity laboratory and worldwide collaborators to address centuries-old questions related to the biodiversity, behavior, and bio-optical properties of organisms living in the water column, from the surface into the deep sea. Many of the most interesting of these phenomena are in the midwater domain—the vast region of ocean between the sunlit surface waters and the deep seafloor.

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Tecnologías ómicas en el género Pleurotus: tendencias a futuro
Guillén Navarro, Griselda Karina ; López Chávez, Mariana Yadira (coaut.) ;
Contenido en: La biología, el cultivo y las propiedades nutricionales y medicinales de las setas Pleurotus spp San Cristóbal de Las Casas, Chiapas, México : El Colegio de la Frontera Sur, 2017 p. 327-345 ISBN:978-607-8429-47-9
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Resumen en español

Las “ómicas” son herramientas de la biología molecular que estudian los fenómenos de una forma global, holística y a gran escala. Con la secuencia genómica de Pleurotus ostreatus se ha logrado deducir, a grandes rasgos, cómo opera su metabolismo y el potencial que tiene para obtener productos útiles; sin embargo, aún existe un importante número de genes y compuestos con función desconocida. Las técnicas transcriptómicas y proteómicas han permitido encontrar genes, transcritos y proteínas de interés biotecnológico relacionadas con la esporulación, fructificación, crecimiento micelial, autolisis del micelio, producción de exopolisacáridos, producción de enzimas lignocelulolíticas y la degradación de xenobióticos, entre otros. Aún son incipientes los estudios de metabolómica del género Pleurotus, pero los resultados encontrados en otros géneros de hongos permiten suponer que tiene un gran potencial para la obtención de compuestos con diferentes propiedades de interés biotecnológico. Estudios integrativos de estas y otras “ómicas” podrían contribuir con la definición de las funciones aún desconocidas de los genes, con el aprovechamiento de productos y metabolitos, y con la comprensión de la fisiología de este hongo.

Resumen en inglés

“OMICS” are tools of molecular biology to study phenomena from a holistic and large scale way. With the genomic sequence of Pleurotus ostreatus it has been possible to deduce roughly how its metabolism works and its potential as a source for useful products; however, there is still a significant number of genes and compounds with unknown function. The transcriptomic and proteomic techniques have allowed the discovery of genes, transcripts and proteins of biotechnological interest related to sporulation, fruiting, mycelial growth, mycelium autolysis, exopolysaccharide production, production of lignocellulolytic enzymes, degradation of xenobiotics, etc. Metabolomics studies of the genus Pleurotus are still emerging, but the results found in other fungal genera suggest a great potential for obtaining compounds with different properties of biotechnological interest. Integrative studies of these and other “omics” could contribute to define the roles of genes still unknown, the use of products and metabolites, and the understanding of the physiology of this mushroom.

Resumen en: Inglés |
Resumen en inglés

It has been demonstrated that the human biomonitoring of susceptible populations is a valuable method for the identification of critical contaminants. Therefore, the purpose of this study was to assess the exposure profile for arsenic (As), lead (Pb), mercury (Hg), 1-hydroxypyrene (1-OHP), 1,1-bis(p-chlorophenyl)-2,2,2-trichloroethane (DDT), 1,1-dichloro-2,2-bis(p-chlorophenyl) ethylene (DDE), polybrominated diphenyl ethers (PBDEs), and polychlorinated biphenyls (PCBs) in children living in Ciudad Juarez, Chihuahua, Mexico (a major manufacturing center in Mexico). In 2012, we evaluated a total of 135 healthy children living in Ciudad Juarez since birth. The total PBDEs levels ranged from nondetectable (< LOD) to 215 ng/g lipid, with a mean total PBDEs level of 29.5 ± 53.0 ng/g lipid (geometric mean ± standard deviation). The mean total PCBs level in the study participants was 29.0 ± 10.5 ng/g lipid (range 4.50–50.0 ng/g lipid). The mean concentration of total DDT (DDT + DDE) was 11.9 ± 6.70 ng/g lipid (range 3.00–26.0 ng/g lipid). The mean 1-OHP levels was 1.2 ± 1.1 µmol/mol creatinine (range <LOD to 3.90 µmol/mol creatinine). Regarding heavy metals levels, the mean urinary As levels was 19.5 ± 3.07 µg/g creatinine, for urinary mercury the levels ranged from <LOD to 11.5 µg/L, with a mean value of 2.10 µg/L, and finally, the mean blood lead level was 4.20 ± 3.80 µg/dL. In conclusion, our data indicate high exposure levels to chemicals analyzed in the children living in the study community. Therefore, a biomonitoring program for the surveillance of the child population in Ciudad Juarez is necessary.

Biochemistry and molecular biology of plants / edited by Bob B. Buchanan, Wilhelm Gruissem and Russell L. Jones
Buchanan, Bob B. (ed.) ; Gruissem, Wilhelm (coed.) ; Jones, Russell L. (coed.) ;
Chichester, West Sussex, United Kingdom : John Wiley & Sons Inc. , 2015
Clasificación: 572.82 / B5
Bibliotecas: Tapachula
SIBE Tapachula
ECO020013684 (Prestado)
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Resumen en inglés

Biochemistry and Molecular Biology of Plants, 2nd Edition has been hailed as a major contribution to the plant sciences literature and critical acclaim has been matched by global sales success. Maintaining the scope and focus of the first edition, the second will provide a major update, include much new material and reorganise some chapters to further improve the presentation. This book is meticulously organised and richly illustrated, having over 1,000 full-colour illustrations and 500 photographs. It is divided into five parts covering: Compartments, Cell Reproduction, Energy Flow, Metabolic and Developmental Integration, and Plant Environment and Agriculture. Specific changes to this edition include: Completely revised with over half of the chapters having a major rewrite. Includes two new chapters on signal transduction and responses to pathogens. Restructuring of section on cell reproduction for improved presentation. Dedicated website to include all illustrative material. Biochemistry and Molecular Biology of Plants holds a unique place in the plant sciences literature as it provides the only comprehensive, authoritative, integrated single volume book in this essential field of study.


The Editors
List of Contributors
About the Companion Website
1 Membrane Structure and Membranous Organelles
1.1 Common properties and inheritance of cell membranes
1.2 The fluid-mosaic membrane model
1.3 Plasma membrane
1.4 Endoplasmic reticulum
1.5 Golgi apparatus
1.6 Exocytosis and endocytosis
1.7 Vacuoles
1.8 The nucleus
1.9 Peroxisomes
1.10 Plastids
1.11 Mitochondria
2 The Cell Wall
2.1 Sugars are building blocks of the cell wall
2.2 Macromolecules of the cell wall
2.3 Cell wall architecture
2.4 Cell wall biosynthesis and assembly
2.5 Growth and cell walls
2.6 Cell differentiation
2.7 Cell walls as sources of food, feed, fiber, and fuel, and their genetic improvement
3 Membrane Transport
3.1 Overview of plant membrane transport systems
3.2 Pumps
3.3 Ion channels
3.4 Cotransporters
3.5 Water transport through aquaporins
4 Protein Sorting and Vesicle Traffic
4.1 The cellular machinery of protein sorting
4.2 Targeting proteins to the plastids
4.3 Targeting proteins to mitochondria
4.4 Targeting proteins to peroxisomes
4.5 Transport in and out of the nucleus
4.6 ER is the secretory pathway port of entry and a protein nursery
4.7 Protein traffic and sorting in the secretory pathway: the ER
4.8 Protein traffic and sorting in the secretory pathway: the Golgi apparatus and beyond
4.9 Endocytosis and endosomal compartments
5 The Cytoskeleton
5.1 Introduction to the cytoskeleton
5.2 Actin and tubulin gene families
5.3 Characteristics of actin filaments and microtubules
5.4 Cytoskeletal accessory proteins
5.5 Observing the cytoskeleton: Statics and dynamics
5.6 Role of actin filaments in directed intracellular movement
5.7 Cortical microtubules and expansion
5.8 The cytoskeleton and signal transduction

5.9 Mitosis and cytokinesis
Cell Reproduction
6 Nucleic Acids
6.1 Composition of nucleic acids and synthesis of nucleotides
6.2 Replication of nuclear DNA
6.3 DNA repair
6.4 DNA recombination
6.5 Organellar DNA
6.6 DNA transcription
6.7 Characteristics and functions of RNA
6.8 RNA processing
7 Amino Acids
7.1 Amino acid biosynthesis in plants: research and prospects
7.2 Assimilation of inorganic nitrogen into N-transport amino acids
7.3 Aromatic amino acids
7.4 Aspartate-derived amino acids
7.5 Branched-chain amino acids
7.6 Glutamate-derived amino acids
7.7 Histidine
8 Lipids
8.1 Structure and function of lipids
8.2 Fatty acid biosynthesis
8.3 Acetyl-CoA carboxylase
8.4 Fatty acid synthase
8.5 Desaturation and elongation of C16 and C18 fatty acids
8.6 Synthesis of unusual fatty acids
8.7 Synthesis of membrane lipids
8.8 Function of membrane lipids
8.9 Synthesis and function of extracellular lipids
8.10 Synthesis and catabolism of storage lipids
8.11 Genetic engineering of lipids
9 Genome Structure and Organization
9.1 Genome structure: a 21st century perspective
9.2 Genome organization
9.3 Transposable elements
9.4 Gene expression
9.5 Chromatin and the epigenetic regulation of gene expression
10 Protein Synthesis, Folding, and Degradation
10.1 Organellar compartmentalization of protein synthesis
10.2 From RNA to protein
10.3 Mechanisms of plant viral translation
10.4 Protein synthesis in plastids
10.5 Post-translational modification of proteins
10.6 Protein degradation
11 Cell Division
11.1 Animal and plant cell cycles
11.2 Historical perspective on cell cycle research
11.3 Mechanisms of cell cycle control
11.4 The cell cycle in action
11.5 Cell cycle control during development

Energy Flow
12 Photosynthesis
12.1 Overview of photosynthesis
12.2 Light absorption and energy conversion
12.3 Photosystem structure and function
12.4 Electron transport pathways in chloroplast membranes
12.5 ATP synthesis in chloroplasts
12.6 Organization and regulation of photosynthetic complexes
12.7 Carbon reactions: the Calvin-Benson cycle
12.8 Rubisco
12.9 Regulation of the Calvin-Benson cycle by light
12.10 Variations in mechanisms of CO 2 fixation
13 Carbohydrate Metabolism
13.1 The concept of metabolite pools
13.2 The hexose phosphate pool: a major crossroads in plant metabolism
13.3 Sucrose biosynthesis
13.4 Sucrose metabolism
13.5 Starch biosynthesis
13.6 Partitioning of photoassimilates between sucrose and starch
13.7 Starch degradation
13.8 The pentose phosphate/triose phosphate pool
13.9 Energy and reducing power for biosynthesis
13.10 Sugar-regulated gene expression
14 Respiration and Photorespiration
14.1 Overview of respiration
14.2 Citric acid cycle
14.3 Plant mitochondrial electron transport
14.4 Plant mitochondrial ATP synthesis
14.5 Regulation of the citric acid cycle and the cytochrome pathway
14.6 Integration of the cytochrome pathway and nonphosphorylating pathways
14.7 Interactions between mitochondria and other cellular compartments
14.8 Biochemical basis of photorespiration
14.9 The photorespiratory pathway
14.10 Role of photorespiration in plants
Metabolic and Developmental Integration
15 LongDistance Transport
15.1 Selection pressures and long-distance transport systems
15.2 Cell biology of transport modules
15.3 Short-distance transport events between xylem and nonvascular cells
15.4 Short-distance transport events between phloem and nonvascular cells
15.5 Whole-plant organization of xylem transport

15.6 Whole-plant organization of phloem transport
15.7 Communication and regulation controlling phloem transport events
16 Nitrogen and Sulfur
16.1 Overview of nitrogen in the biosphere and in plants
16.2 Overview of biological nitrogen fixation
16.3 Enzymology of nitrogen fixation
16.4 Symbiotic nitrogen fixation
16.5 Ammonia uptake and transport
16.6 Nitrate uptake and transport
16.7 Nitrate reduction
16.8 Nitrite reduction
16.9 Nitrate signaling
16.10 Interaction between nitrate assimilation and carbon metabolism
16.11 Overview of sulfur in the biosphere and plants
16.12 Sulfur chemistry and function
16.13 Sulfate uptake and transport
16.14 The reductive sulfate assimilation pathway
16.15 Cysteine synthesis
16.16 Synthesis and function of glutathione and its derivatives
16.17 Sulfated compounds
16.18 Regulation of sulfate assimilation and interaction with nitrogen and carbon metabolism
17 Biosynthesis of Hormones
17.1 Gibberellins
17.2 Abscisic acid
17.3 Cytokinins
17.4 Auxins
17.5 Ethylene
17.6 Brassinosteroids
17.7 Polyamines
17.8 Jasmonic acid
17.9 Salicylic acid
17.10 Strigolactones
18 Signal Transduction
18.1 Characteristics of signal perception, transduction, and integration in plants
18.2 Overview of signal perception at the plasma membrane
18.3 Intracellular signal transduction, amplification, and integration via second messengers and MAPK cascades
18.4 Ethylene signal transduction
18.5 Cytokinin signal transduction
18.6 Integration of auxin signaling and transport
18.7 Signal transduction from phytochromes
18.8 Gibberellin signal transduction and its integration with phytochrome signaling during seedling development
18.9 Integration of light, ABA, and CO2 signals in the regulation of stomatal aperture
18.10 Prospects

19 Molecular Regulation of Reproductive Development
19.1 The transition from vegetative to reproductive development
19.2 The molecular basis of flower development
19.3 The formation of male gametes
19.4 The formation of female gametes
19.5 Pollination and fertilization
19.6 The molecular basis of selfincompatibility
19.7 Seed development
20 Senescence and Cell Death
20.1 Types of cell death
20.2 PCD during seed development and germination
20.3 Cell death during the development of secretory bodies, defensive structures and organ shapes
20.4 PCD during reproductive development
20.5 Senescence and PCD in the terminal development of leaves and other lateral organs
20.6 Pigment metabolism in senescence
20.7 Macromolecule breakdown and salvage of nutrients in senescence
20.8 Energy and oxidative metabolism during senescence
20.9 Environmental influences on senescence and cell death I: Abiotic interactions
20.10 Environmental influences on senescence and cell death II: PCD responses to pathogen attack
20.11 Plant hormones in senescence and defense-related PCD
Plant Environment and Agriculture
21 Responses to Plant Pathogens
21.1 Pathogens, pests, and disease
21.2 An overview of immunity and defense
21.3 How pathogens and pests cause disease
21.4 Preformed defenses
21.5 Induced defense
21.6 Effector-triggered immunity, a second level of induced defense
21.7 Other sources of genetic variation for resistance
21.8 Local and systemic defense signaling
21.9 Plant gene silencing confers virus resistance, tolerance, and attenuation
21.10 Control of plant pathogens by genetic engineering
22 Responses to Abiotic Stress
22.1 Plant responses to abiotic stress
22.2 Physiological and cellular responses to water deficit

22.3 Gene expression and signal transduction in response to dehydration
22.4 Freezing and chilling stress
22.5 Flooding and oxygen deficit
22.6 Oxidative stress
22.7 Heat stress
22.8 Crosstalk in stress responses
23 Mineral Nutrient Acquisition, Transport, and Utilization
23.1 Overview of essential mineral elements
23.2 Mechanisms and regulation of plant K+ transport
23.3 Phosphorus nutrition and transport
23.4 The molecular physiology of micronutrient acquisition
23.5 Plant responses to mineral toxicity
24 Natural Products
24.1 Terpenoids
24.2 Biosynthesis of the basic five-carbon unit
24.3 Repetitive additions of C5 units
24.4 Formation of parent carbon skeletons
24.5 Modification of terpenoid skeletons
24.6 Metabolic engineering of terpenoid production
24.7 Cyanogenic glycosides
24.8 Cyanogenic glycoside biosynthesis
24.9 Functions of cyanogenic glycosides
24.10 Glucosinolates
24.11 Alkaloids
24.12 Alkaloid biosynthesis
24.13 Biotechnological application of alkaloid biosynthesis research
24.14 Phenolic compounds
24.15 Phenolic biosynthesis
24.16 The phenylpropanoid-acetate pathway
24.17 The phenylpropanoid pathway
24.18 Universal features of phenolic biosynthesis
24.19 Evolution of secondary pathways
Further reading

Tesis - Maestría
Determinación de plaguicidas organoclorados y su relación con parámetros sanguíneos en tortugas Caguama (Caretta caretta (Linnaeus, 1758)) de Quintana Roo, México / Dulce Thelma González Castillo
González Castillo, Dulce Thelma ; Álvarez Legorreta, Teresa (directora) ; Torres Dosal, Arturo (asesor) ; Zapata Pérez, José Omar (asesor) ;
Chetumal, Quintana Roo, México : El Colegio de la Frontera Sur , 2015
Clasificación: TE/597.928097267 / G6
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ECO030008267 (Disponible)
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Índice | Resumen en: Español |
Resumen en español

El objetivo de este trabajo fue determinar la concentración de 16 plaguicidas organoclorados (POC) en plasma sanguíneo de tortugas marinas anidadoras de caguama (Caretta caretta), que se relacionaron con los valores obtenidos de la bioquímica sanguínea y hemograma (parámetros sanguíneos). Para la determinación de los POC, se colectaron muestras de sangre en 37 tortugas que anidadoron en la playa Aventuras-DIF, una de las principales zonas de anidación de esta especie en Quintana Roo, México. Adicionalmente, se obtuvieron 26 muestras sanguíneas para determinar siete valores de bioquímica sanguínea (aspartato amino transferesa o AST, lactato deshidrogenasa o LDH, glucosa, albumina, ácido úrico, urea nitrógeno o BUN y urea) y 11 para hemograma (glóbulos rojos o RBC, glóbulos blancos o WBC, hematocrito o HTO, linfocitos, heterófilos, monocitos, eosinófilos y basófilos). Las concentraciones promedio de POC más altas, de manera individual, estuvieron dadas por el β-HCH (4.97ng/ml) y p,p´-DDT (4.87 ng/ml), y por sumatoria de familia, fueron la ƩHCH (6,85 ng/ml) y Ʃheptacloros (2.85 ng/ml); las concentraciones más bajas estuvieron dadas por endrín aldehído (0.93 ng/ml), p,p´-DDD (0.94 ng/ml) y p,p´-DDE (1.06 ng/ml), y por sumatoria de familia la ƩDDT´s (2.13 ng/ml).

Estas concentraciones de POC fueron consideradas como relativamente bajas comparadas con otros estudios que consideran esto mismo para otras poblaciones de tortugas marinas. Se encontró que la mayoría de los parámetros de la bioquímica sanguínea (AST y LDH, urea, BUN) y del hemograma estuvieron cercanos a los promedios o dentro de los rangos reportados para otras tortugas de diferentes regiones geográficas del mundo y aparentemente saludables o en estado de anidación normal; únicamente los valores de glucosa, albumina, ácido úrico y los basófilos se mostraron diferentes. Se encontraron correlaciones entre algunos POC con los parámetros sanguíneos de manera positiva o negativa (glucosa ↓, albumina ↓, LDH ↓, RBC ↑, heterófilos ↓, linfocitos ↑, eosinófilos ↑, monocitos ↑ y basófilos ↑). Estas correlaciones no demuestran causa-efecto, pero sugieren que las variaciones de las sustancias corporales posiblemente están siendo moduladas por los POC y que, al parecer, las tortugas marinas son sensibles fisiológicamente a bajas concentraciones de los POC.


Materiales y métodos
Área de estudio
Muestreo y registro de datos
Plaguicidas organoclorados (POC)
Bioquímica sanguínea
Análisis estadístico
Área de estudio, muestreo y mediciones
Determinación de POC
Bioquímica sanguínea y hemograma (parámetros sanguíneos)
Relación entre las concentraciones de POC y los parámetros sanguíneos
Determinación de plaguicidas organoclorados (POC)
Bioquímica sanguínea y hemograma (parámetros sanguíneos)
Relación entre los plaguicidas organoclorados (POC) con los parámetros sanguíneos
Literatura citada

Tesis - Maestría
Estudio molecular de dos poblaciones de Artibeus jamaicensis (Chiroptera: Phyllostomidae) / Viridiana Llaven Macías
Llaven Macías, Viridiana ; Machkour M'Rabet, Salima (directora) ; Ruiz Montoya, Lorena (asesora) (1964-) ; Lesher Gordillo, Julia María (asesora) ;
Chetumal, Quintana Roo, México : El Colegio de la Frontera Sur , 2015
Clasificación: TE/599.45097275 / L4
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Capítulo I
Introducción general
Capítulo II
Palabras clave
Keys words
Materiales y métodos
Literatura citada
Capítulo III
Conclusiones generales
Capítulo IV
Literatura citada

Insect molecular biology and ecology / editor Klaus H. Hoffman
Hoffman, Klaus Hubert (ed.) (1946-) ;
Boca Raton, Florida : CRC Press :: Taylor & Francis Group , c2015
Clasificación: 595.7 / I53
Bibliotecas: Chetumal , Tapachula
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Índice | Resumen en: Inglés |
Resumen en inglés

Insects represent the most abundant and diverse animal group on Earth. The number of described species is more than one million and up to ten million are estimated. Insects have one of the widest distributions in the world because they have adapted to extreme ranges of environments. Molecular ecology studies ecological processes based on the analysis of biomacromolecules, particularly DNA, RNA, and proteins, but also of low-molecular weight signal compounds. Molecular ecology uses the exciting opportunities offered by the tools of molecular biology. The book presents current entomological research, where molecular tools help to advance traditional ecological studies. Chapters include ones on insect–insect and insect–plant interactions, on mechanisms of environmental adaptation, or on the use of insect biotechnology in pest and vector control. The book helps to combine powerful methods in molecular biology with exciting issues in ecology to understand why insects became "masters of survival."


1. Mechanisms of Polyphenism in Insects
2. Toxins, Defensive Compounds and Drugs from Insects
3. Insect Bioluminescence in the Post-Molecular Biology Era
4. A Glance on the Role of miRNAs in Insect Life
5. Advances in Insect Physiology and Endocrinology Through Genomics and Peptidomics
6. Neuropeptide Signaling and RNA Interference
7. Insect Photoperiodism
8. Insects in Winter: Metabolism and Regulation of Cold Hardiness
9. Evolutionary Ecology of Insect Immunity
10. The Coleopteran Gut and Targets for Pest Control
11. Trypsin Modulating Oostatic Factor (TMOF) and Insect Biotechnology
12. RNAi Based Functional Analysis of Biosynthetic Enzymes and Transport Proteins Involved in the Chemical Defense of Juvenile Leaf Beetles
13. Silks from Insects-From Natural Diversity to Applications
Insect Index
Subject Index
Color Plate Section

Biochemical ecotoxicology: principles and methods / Francois Gagné
Gagné, Francois ;
Amsterdam : Academic Press , 2014
Clasificación: 571.85 / G3
Bibliotecas: Campeche
SIBE Campeche
ECO040005709 (Disponible)
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Resumen en inglés

Biochemical Ecotoxicology: Principles and Methods presents practical approaches to biochemical ecotoxicology experiments for environmental protection and conservation. With its methodical, stepped approach this essential reference introduces readers to current techniques for toxicity endpoint testing, suitable for laboratories of any size and budget. Each chapter presents a state-of-the-art principle, a quick and inexpensive procedure (including appropriate reagents), case studies, and demonstrations on how to analyze your results. Generic techniques are covered, suitable for a variety of organisms, as well as high-throughput techniques like quantitative polymerase chain reactions and enzyme-linked immunoassays. Cutting-edge approaches, including gPCR arrays and lipidomic techniques, are also included, making this is an essential reference for anyone who needs to assess environmental toxicity.


List of Contributors
1. Quantitative Assessments of Biochemical Assesments
2. Tissue Preparation and Subcellular Fractionation Techniques
3. Preparation and Maintenance of Live Tissues and Primary Cultures for Toxicity Studies
4. Measuring Effects at the Gene Expression Level
5. Metal Metabolism and Detoxication
6. Oxidative Stress
7. Xeno Biotransformation
8. Cellular Energy Allocation
9. Neuroendocrine Disruption
10. Genotoxicity
11. Biomarkers of Infection and Diseases
12. Descriptive and Analytical Statistics in Biochemical Ecotoxicology
13. Integration of Biomarkers into Indices