Términos relacionados

98 resultados encontrados para: TEMA: Ceratitis capitata
  • «
  • 1 de 10
  • »
1.
Libro
Programa mosca del mediterráneo: manual de operación
Distrito Federal, México : Secretaría de Agricultura y Recursos Hidráulicos. Dirección General de Sanidad Vegetal , s.f.
Clasificación: F/632.774 / M4
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020008398 (Disponible)
Disponibles para prestamo: 1

2.
- Artículo con arbitraje
*Solicítelo con su bibliotecario/a
Suppression of Ceratitis capitata (Wied.) (Diptera: Tephritidae) populations in coffee in the Mexico–Guatemala border region through the augmentative releases of Diachasmimorpha longicaudata (Ashmead) (Hymenoptera: Braconidae)
Cancino Díaz, Jorge Luis (autor) ; Ruiz, Lía (coaut.) ; López, Elmer (autor) ; Aguilar, Emilio (autor) ; Gálvez, César (autor) ; Montoya Gerardo, Pablo Jesús (autor) ; Liedo Fernández, Pablo (autor) ;
Disponible en línea
Contenido en: Biocontrol Science and Technology Vol. 29, no. 8 (August 2019), p. 822-826 ISSN: 1360-0478
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

In Guatemalan coffee plantations, Ceratitis capitata populations were suppressed by the application of augmentative releases of parasitoids. These releases significantly increased parasitism of this fruit fly over paired no-release control areas. Integrated management plus biological control presented a significant reduction in the pest population. These results support the application of biological control in C. capitata management.


PDF PDF
Resumen en: Inglés |
Resumen en inglés

The transgenic strain of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wied.) VIENNA 8 1260, developed from the classical genetic sexing strain VIENNA 8, has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testicles and sperm. These traits offer a precise tool to discriminate between mass-reared sterile males and wild fertile males, and they could potentially increase the effectiveness of control programs for this pest. To assess the risk of horizontal transfer of the fluorescence transgenes in natural ecosystems, we used the VIENNA 8 1260 strain and the medfly parasitoid Fopius ceratitivorus. The fluorescence signal and the inheritance of the fluorescence gene markers were monitored for over 16 generations (about two years) in both species using fluorescence microscopy and a PCR-based assay. The PCR analysis was performed in four independent laboratories. Both fluorescence microscopy and PCR analysis indicated that no horizontal gene transfer of the DsRed transgene occurred during 16 generations of medfly parasitoid rearing under experimental conditions.


4.
Tesis - Doctorado
Desarrollo de un modelo predictivo del crecimiento de poblaciones de mosca del Mediterráneo / Salvador Flores Breceda
Flores Breceda, Salvador (autor) ; Liedo Fernández, Pablo (director) ; Enkerlin Hoeflich, Walter Raúl (asesor) ; Ruiz Montoya, Lorena (asesora) (1964-) ; Montoya Gerardo, Pablo Jesús (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2017
Disponible en línea
Clasificación: TE/632.774097281 / F4
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013670 (Disponible)
Disponibles para prestamo: 1
PDF
Índice | Resumen en: Español |
Resumen en español

La mosca del Mediterráneo, Ceratitis capitata (Diptera, Tephritidae), es una plaga originaria de África con un alto potencial invasivo, que se encuentra establecida en Guatemala. Esta plaga presenta variaciones poblacionales asociadas a fenómenos climáticos globales que provocan cambios en las temperaturas, precipitaciones, los cuales tienen repercusión en la fenología de sus hospedantes. El poder predecir el nivel poblacional de la mosca representaría un apoyo en la programación de las actividades de control. En este trabajo se desarrolló un modelo predictivo de la fluctuación poblacional de C. capitata, y se generó información sobre la diversidad y el flujo genético entre las poblaciones de diferentes regiones de Guatemala. También se determinó la fluctuación poblacional de la especie en áreas donde no se realizan actividades de control, y la duración del ciclo biológico a diferentes altitudes. El análisis genético indicó una diversidad moderada (Ho = 0.28) y una diferenciación leve entre poblaciones. Los resultados de la estructura genética se agruparon a las poblaciones de la región occidente y a las poblaciones del oriente de Guatemala, lo cual fue concordante con el análisis filogenético.

La fluctuación poblacional se asoció con la disponibilidad de hospedantes, temperatura y precipitación. La densidad poblacional se incrementó en relación directa con la altitud y la abundancia del café y cítricos como naranja y mandarina. La duración del ciclo de vida fue regulada por la temperatura, y su duración fue estimada con precisión empleando los umbrales y requerimientos térmicos de los modelo de Tassan. En el modelo de estimación poblacional en cafetales o áreas marginales se emplearon como variables la temperatura media, mínima y máxima, precipitación, la densidad inicial y la presencia del hospedante primario. Este modelo se ajustó a los datos en un 70% durante el periodo de estudio, con una fluctuación cíclica de las poblaciones. Un aumento de temperatura en 0.2 °C representa cambios mas aparentes que con un 10% de incremento en la precipitación. Sin embargo, es necesario realizar validación del modelo en áreas infestadas bajo diferentes condiciones ambientales.

Índice

Resumen
Capítulo 1
Introducción
Capítulo 2
Genetic diversity and structure of Ceratitis capitata populations in Guatemala
Capítulo 3
Population Fluctuation of Ceratitis capitata (Diptera: Tephritidae) as a Function of Altitude in Eastern Guatemala
Capítulo 4
Desarrollo y sobrevivencia de Ceratitis capitata a diferentes altitudes en el Este de Guatemala: Comparación de dos modelos térmicos
Capítulo 5
Modelo para la predicción de poblaciones de Ceratitis capitata en áreas cafetaleras del Oriente de Guatemala
Capítulo 6
Discusión general
Anexos


5.
Artículo
*Solicítelo con su bibliotecario/a
Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
Ramírez Santos, Edwin Mauricio ; Rendón Arana, Pedro Alfonso (coaut.) ; Ruiz Montoya, Lorena (coaut.) (1964-) ; Toledo, Jorge (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Journal of Economic Entomology Vol. 110, no. 4 (August 2017), p. 1483–1494 ISSN: 1938-291X
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

The genetically modified strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) VIENNA 8 1260, was developed from the genetic sexing strain VIENNA 8. It has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testis and sperm. These traits offer a precise tool to discriminate between mass-reared and wild males, increasing the effectiveness of sterile insect technique. The reproductive performance of the VIENNA 8 1260 and VIENNA 8 D53- (without the D53 inversion introduced to prevent recombination) was compared at different irradiation doses. The general effect of irradiation on VIENNA 8 1260 followed the same patterns documented in previous publications for VIENNA 8 D53-. Irradiation doses of 80 Gray or greater reduced fertility and induced high levels of sterility in wild females. Fecundity reduction was higher in VIENNA 8 1260 than in VIENNA 8 D53- females. Vertical transmission of the fluorescence gene was confirmed up to the F4 generation. Substerilization in the VIENNA 8 1260 could jeopardize the usefulness of the transgenic strain due to the possible vertical transfer of the fluorescence transgene from the sterile males to the wild flies. A biologically safe higher irradiation dose could result in reduced competitiveness of the VIENNA 8 1260 strain. Mating and remating experiments suggest that Mediterranean fruit fly females exhibit a relative precedence in the use of the sperm: though both sperms are mixed, sperm from the remating is spent first. Results suggest a lower fitness of VIENNA 8 1260 sperm, when compared with sperm from a nonfluorescent bisexual strain, which is consistent with the lower reproductive performance documented for the VIENNA 8 1260 strain.


Resumen en: Inglés |
Resumen en inglés

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is considered one of the most important pests worldwide because of its direct damage to fruit and vegetable production, and restrictions imposed to commercialization of horticultural commodities by countries free of the pest. It was introduced to Brazil in 1901 and to Costa Rica in 1955, from where it spread across the Central American region, reaching Guatemala and Mexico in 1976 and 1977, respectively. In response, the governments of Guatemala, Mexico, and the USA joined efforts to (1) contain further northward spread of the pest, (2) eradicate it from the areas it had invaded in southernMexico, and (3) in the longer termeradicate it from Guatemala and eventually from the rest of Central America. To this effect, cooperative agreements were subscribed between the three countries and also between the USA and Belize. This allowed regional cooperation against the Mediterranean fruit fly and the creation of the Moscamed Programme. The programme was the first area-wide large-scale application of the sterile insect technique (SIT) against this pest. By 1982, the Programme had achieved its first two objectives with the containment of the northward advance of the pest, and its eradication from the areas it had invaded in the states of Chiapas and Oaxaca in southern Mexico. Furthermore, by 1985 the Mediterranean fruit fly had been eradicated from areas in Guatemala located at the border with Mexico. Since then, the programme has had years with significant territorial advances in the eradication of the pest from areas within Guatemala, combined with years when it had setbacks resulting in losses of the territorial gains. Nevertheless, during 4 decades, the programme has effectively served as an effective containment barrier maintaining the Mediterranean fruit fly-free status of Belize, Mexico, and the USA.

It has also protected and increased the Mediterranean fruit fly-free areas in Guatemala. As a result, it has protected the assets of horticultural producers and contributed during this period to the development of multibillion dollar export industries in these countries. This paper provides an historical review of the programme and describes briefly how technological innovations and decision-making tools have contributed to programme efficiency. It also discusses how non-technical and external factors have limited the eradication process and further programme advance within the Central American region.


7.
Artículo
*Solicítelo con su bibliotecario/a
Pathogenicity of three formulations of Beauveria bassiana and efficacy of autoinoculation devices and sterile fruit fly males for dissemination of conidia for the control of Ceratitis capitata
Toledo, Jorge ; Flores Breceda, Salvador (coaut.) ; Campos, Sergio (coaut.) ; Villaseñor Cortés, Antonio (coaut.) ; Enkerlin Hoeflich, Walther Raúl (coaut.) ; Liedo Fernández, Pablo (coaut.) ; Valle, Álvaro (coaut.) ; Montoya Gerardo, Pablo Jesús (coaut.) ;
Contenido en: Entomologia Experimentalis et Applicata Vol. 164, no. 3 (September 2017), p. 340–349 ISSN: 1570-7458
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

This study reports the pathogenicity of three formulations of Beauveria bassiana (Balsamo) Vuillemin (Hypocreales) – Bb-ET, GHA, and Bb-AES – and their application in panel-type and cylinder-type autoinoculation devices, and using sterile males as vectors for the control of Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) in coffee-producing areas of the Central Highlands of Guatemala. Using sterile adults of C. capitata under laboratory bioassays, mean lethal concentrations (LC50) of 1.4 9 107, 2.4 9 106, and 8.2 9 105 conidia ml-¹, and median survival times of 4.1 0.1, 4.2 0.1, and 3.8 0.1 days, were recorded for the strains GHA, Bb-AES, and Bb-ET, respectively. These values indicate that the three strains were sufficiently pathogenic and that their period of biological activity was considered adequate for using sterile flies as vectors of the inoculum. Observed percentages of sporulated wild C. capitata flies were 57.3, 44.7, and 44.3% for sterile fly vectors, panel devices, and cylinder devices, respectively. The total population reduction at the end of the study period was over 90% for the three treatments. Our results show that sterile males used as vectors or disseminator devices may represent a new control method for area-wide integrated management of Mediterranean fruit flies. The potential of this new approach and its integration with other control methods, including the sterile insect technique, is discussed.


8.
- Artículo con arbitraje
*Solicítelo con su bibliotecario/a
Performance of a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae) for area-wide integrated pest management with the sterile insect technique
Ramírez Santos, Edwin Mauricio ; Rendón Arana, Pedro Alfonso (coaut.) ; Ruiz Montoya, Lorena (coaut.) (1964-) ; Toledo, Jorge (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Biological and Microbial Control Vol. 110, no. 1 (February 2017), p. 24–34 ISSN: 1938-291X
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

The genetically modified strain of Ceratitis capitata (Wiedemann) VIENNA 8 1260 has two morphological markers that exhibit fluorescence in body and sperm. To assess the feasibility of its use in area-wide integrated pest management (AW-IPM) programs using the sterile insect technique, its rearing performance and quality control profile under small, medium, and large scales was evaluated, as well as in field cages. The VIENNA 8 1260 strain had a lower yield than the control strains, VIENNA 8 with D53 inversion (VIENNA 8) and without D53 inversion (VIENNA 8 D53-). At mass-rearing scale, yield gradually increased in three generations without reaching the control strain values. The VIENNA 8 1260 strain was stable in the genetic sexing mechanism (>99.9%) and expression of fluorescence (100%). In field cages, the VIENNA 8 1260 males reduced the mating potential of wild males in the same magnitude as the VIENNA 8, when evaluated in independent cage tests. However, the relative sterility index and the strain male relative performance index of VIENNA 8 1260 males were significantly lower than those of the VIENNA 8. There were no significant differences in longevity of these strains. The potential application of the VIENNA 8 1260 in AW-IPM programs is further discussed.


9.
Tesis - Doctorado
Desempeño de una cepa genéticamente modificada de la mosca del Mediterráneo, Ceratitis capitata (Wied.) para su uso en la TIE / Edwin Mauricio Ramírez Santos
Ramírez Santos, Edwin Mauricio (autor) ; Liedo Fernández, Pablo (director) ; Ruiz Montoya, Lorena (asesora) (1964-) ; Rendón Arana, Pedro Alfonso (asesor) ; Toledo, Jorge (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2016
Clasificación: TE/632.774 / R34
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013581 (Disponible)
Disponibles para prestamo: 1
PDF
Índice | Resumen en: Español |
Resumen en español

La utilización de organismos genéticamente modificados puede contribuir a mejorar la eficiencia de la Técnica del Insecto Estéril (TIE). Recientemente se han desarrollado cepas de la mosca del Mediterráneo, que portan secuencias de ADN que codifican para la expresión de proteínas fluorescentes. Una de estas cepas fluorescentes es la #1260_F-3_m-1 (VIENNA 8 1260), desarrollada a partir de la cepa VIENNA 8, que porta genes que permiten la separación de hembras y machos. La expresión de fluorescencia, producto de la inserción, puede ser aprovechada como marcador para dar mayor eficiencia a la TIE, al identificar en campo de forma precisa el origen de los individuos. Por ser insectos transgénicos el uso de la cepa VIENNA 8 1260 puede tener un costo biológico en su desempeño, el riesgo de transmitir el transgen de fluorescencia a poblaciones silvestres de esta especie y debe cumplir con requisitos de bioseguridad para su liberación en campo. Se realizó una serie de ensayos para determinar la viabilidad del uso de la cepa transgénica en programas que aplican la TIE. Se documentó una mejora generacional, gradual, en el desempeño de la cepa en cría masiva, alta estabilidad y conservación de la fluorescencia del 100%. La competitividad sexual bajo condiciones de jaula de campo fue menor para VIENNA 8 1260 que para la cepa actualmente en uso (VIENNA 8 D53-). Las dosis de irradiación requeridas para la esterilización fueron similares para ambas cepas; sin embargo, para minimizar el riesgo de la trasmisión vertical hacia la población silvestre, se recomiendan dosis mayores a 140 Gy. Se evidenció recópula en hembras, precedencia en el uso de esperma a favor del macho con que recopulan y un mayor desempeño (fitness) del esperma de machos no fluorescentes. No se observó trasmisión horizontal del transgen fluorescente hacia el parasitoide Fopius ceratitivorus durante 16 generaciones.

Índice

Resumen
Capítulo I Introducción
Capítulo II Desempeño de una cepa genéticamente modificada de la mosca de la fruta, Ceratitis capitata (Wied.) para su uso en un programa de manejo integrado de plagas en áreas extensas (MIP-AE) que emplea la técnica del insecto estéril (TIE)
Capítulo III Efectos de la dosis de irradiación sobre la inducción de esterilidad y garantías biológicas de una cepa genéticamente modificada de la mosca mediterránea de la fruta, Ceratitis capitata (Wied.)
Capítulo IV El uso de la PCR como una herramienta de evaluación de la transferencia horizontal de marcadores de ADN entre una cepa genéticamente modificada de la mosca mediterránea de la fruta, Ceratitis capitata (Wied.) y su parasitoide específico Fopius ceratitivorus Wharton (Braconidae)
Capítulo V Conclusiones generales
Literatura citada
Anexos


10.
- Artículo con arbitraje
*Solicítelo con su bibliotecario/a
Population fluctuation of Ceratitis capitata (Diptera: Tephritidae) as a function of altitude in eastern Guatemala
Flores Breceda, Salvador (coaut.) ; Montoya Gerardo, Pablo Jesús (coaut.) ; Ruiz Montoya, Lorena (coaut.) (1964-) ; Villaseñor, A. (coaut.) ; Valle, A. (coaut.) ; Enkerlin Hoeflich, Walther Raúl (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Environmental Entomology Vol. 45, no. 4 (August 2016), p. 802-811 ISSN: 1938-2936
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

Population fluctuations of Ceratitis capitata (Wiedemann) were evaluated over a period of 12 mo in four altitudinal strata (400–750, 750–1,100, 1,100–1,450, and 1,450–1,800 meters above sea level, masl) in Eastern Guatemala. Within each altitudinal range, sampling plots were established in coffee plantations and adjacent areas, in which Jackson traps were set and baited with Trimedlure. Coffee berries and other host fruits were collected. Population density was lowest at the 400–750 masl stratum and highest at 1,450–1,800 masl. At every altitudinal range, the fluctuations of the pest were associated mainly with the availability of ripe coffee berries as a primary host. From 750–1,450 masl, the pest was also associated with the availability of sweet orange and mandarins in commercial and backyard orchards. The highest densities of the pest were recorded in the dry season. Citrus were the main alternate host where ripe coffee berries were not available. This knowledge on population dynamics of C. capitata will contribute to develop more effective area-wide pest management strategies including the use of sterile insects, natural enemies, and bait sprays.