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No. de sistema: 000009553

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008 _ _ 200907m20209999xx^^r^p^o^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
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245 0 0 a| Chemical composition of an anthelmintic fraction of Pleurotus eryngii against eggs and infective larvae (L3) of Haemonchus contortus
506 _ _ a| Acceso en línea sin restricciones
520 1 _ a| This study was aimed at evaluating thein vitroeffect of the edible mushroom (EM) Pleurotus eryngiiagainst the eggs and larvae (L3) of Haemonchus contortus. The evaluation included acetone (AE) and hydroalcoholic (HA) extracts of the following strains: ECS-1138, ECS-1156, ECS-1255, ECS-1258, ECS-1261, ECS-1282, and ECS-1292. The HA extract of the ECS-1255 strain showed thehighest effect on mortality rates of L3 (18.83%) at 20 μg/mL. After subjecting this HA extract to a normal phase chromatography column, five fractions were obtained; fraction F5 (100% MeOH) was the most effective against eggs, with hatching inhibition percentages of 88.77 and 91.87% at 20 and 40 mg/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) subjected this fraction to an acetylation reaction to determine the content of the secondary metabolites. The GC-MSanalysis showed that the F5 fraction was composed of trehalose CAS: 6138-23-4, polyols (L-iditol CAS: 488-45-9, galactitol CAS:608-66-2, D-mannitol CAS: 69-65-8, D-glucitol CAS: 50-70-4, and myoinositol CAS: 87-89-8), adipic acid CAS: 124-04-9, stearic acid CAS: 57-11-4, squalene CAS: 111-02-4, and β-sitosterol CAS: 83-46-5.
530 _ _ a| Disponible en línea
533 _ _ a| Reproducción electrónica en formato PDF
538 _ _ a| Adobe Acrobat profesional 6.0 o superior
650 _ 4 a| Pleurotus eryngii
650 _ 4 a| Hongos comestibles
650 _ 4 a| Composición química
650 _ 4 a| Haemonchus contortus
650 _ 4 a| Helmintos
650 _ 4 a| Ovejas
650 _ 4 a| Control de nemátodos
700 1 _ a| Cruz Arévalo, Julio
e| autor
700 1 _ a| Sánchez, José E.
e| autor
n| 55452597200
700 1 _ a| González Cortázar, Manasés
e| autor
700 1 _ a| Zamilpa, Alejandro
e| autor
700 1 _ a| Andrade Gallegos, René Humberto
c| Mtro.
e| autor
700 1 _ a| Mendoza de Gives, Pedro
e| autor
700 1 _ a| Aguilar Marcelino, Liliana
e| autora
773 0 _
t| BioMed Research International
g| Volumen 2020, artículo ID 4138950 (August 2020), p. 1-8
x| 2314-6141
856 4 1 u| https://www.hindawi.com/journals/bmri/2020/4138950/
z| Artículo electrónico
902 _ _ a| BG / MM
904 _ _ a| Septiembre 2020
905 _ _ a| Artecosur
905 _ _ a| Biblioelectrónica
LNG eng
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Chemical composition of an anthelmintic fraction of Pleurotus eryngii against eggs and infective larvae (L3) of Haemonchus contortus
Cruz Arévalo, Julio (autor)
Sánchez, José E. (autor)
González Cortázar, Manasés (autor)
Zamilpa, Alejandro (autor)
Andrade Gallegos, René Humberto (autor)
Mendoza de Gives, Pedro (autor)
Aguilar Marcelino, Liliana (autora)
Nota: Disponible en línea
Acceso en línea sin restricciones
Contenido en: BioMed Research International. Volumen 2020, artículo ID 4138950 (August 2020), p. 1-8. ISSN: 2314-6141
No. de sistema: 9553
Tipo: Artículo
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Inglés

"This study was aimed at evaluating thein vitroeffect of the edible mushroom (EM) Pleurotus eryngiiagainst the eggs and larvae (L3) of Haemonchus contortus. The evaluation included acetone (AE) and hydroalcoholic (HA) extracts of the following strains: ECS-1138, ECS-1156, ECS-1255, ECS-1258, ECS-1261, ECS-1282, and ECS-1292. The HA extract of the ECS-1255 strain showed thehighest effect on mortality rates of L3 (18.83%) at 20 μg/mL. After subjecting this HA extract to a normal phase chromatography column, five fractions were obtained; fraction F5 (100% MeOH) was the most effective against eggs, with hatching inhibition percentages of 88.77 and 91.87% at 20 and 40 mg/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) subjected this fraction to an acetylation reaction to determine the content of the secondary metabolites. The GC-MSanalysis showed that the F5 fraction was composed of trehalose CAS: 6138-23-4, polyols (L-iditol CAS: 488-45-9, galactitol CAS:608-66-2, D-mannitol CAS: 69-65-8, D-glucitol CAS: 50-70-4, and myoinositol CAS: 87-89-8), adipic acid CAS: 124-04-9, stearic acid CAS: 57-11-4, squalene CAS: 111-02-4, and β-sitosterol CAS: 83-46-5."


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