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No. de sistema: 000009804

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 161124m20169999xx^mr^p^^^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
043 _ _ a| n-mx-oa
044 _ _ a| xx
245 0 0 a| Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues
520 1 _ a| Nuclear mitochondrial (mt) paralogues (numts) are non-functional fragments of mtDNA integrated into the nuclear genome that can overestimate the number of species in analyses based on mtDNA sequences. As numts have relatively slow mutation rates, they can pass undetected by conventional procedures such as inspecting for internal stop codons, indels or apparent polymorphism in chromatograms. Species boundaries based on mtDNA markers therefore require a thorough assessment of numts, especially in insects, where this phenomenon appears to be relatively frequent. Ectatomma ruidum is a widely distributed Neotropical ant species that is distributed from northern Mexico to northern Brazil. Previous behavioural and molecular evidence suggests that this species actually represents a composite taxon. Here we assessed the species boundaries in E. ruidum based on two mt (COI, cyt b) and one nuclear (H3) marker, as well as on external morphology. Ancient and recent mt paralogues were detected in several specimens, although pre-PCR dilution of DNA template helped to recover most of the mt orthologues. Based on the congruence found between our species delineation obtained from the mt genealogies and the discriminated morphospecies, we propose that E. ruidum is actually composed of at least three species. Two of these species have a wide geographical distribution in the Neotropics, whereas the remaining one was restricted to localities situated near the Pacific coast in south-east Mexico. We also found extensive intra- and interspecific variation in the barcoding locus. Moreover, the nuclear evidence suggests the existence of hybrids between two of these species in Oaxaca, south-east Mexico. This study agrees with previous studies of other closely related animal taxa, which have revealed a complex evolutionary history and overlooked species diversity in the latter region.
650 _ 4 a| Ectatomma ruidum
650 _ 4 a| Hibridación
650 _ 4 a| Taxonomía animal
650 _ 4 a| Ácido desoxirribonucléico
650 _ 4 a| Zoogeografía
651 _ 4 a| Oaxaca (México)
700 1 _ a| Aguilar Velasco, Reina Gabriela
700 1 _ a| Poteaux, Chantal
e| coaut.
700 1 _ a| Meza Lázaro, Rubí
e| coaut.
700 1 _ a| Lachaud, Jean Paul
e| coaut.
700 1 _ a| Dubovikoff, Dmitry A.
e| coaut.
n| 54906211400
700 1 _ a| Zaldívar Riverón, Alejandro
e| coaut.
773 0 _
t| Zoological Journal of the Linnean Society
g| Vol. 178, no. 2 (October 2016), p. 226–240
x| 1096-3642
856 4 1 u| http://onlinelibrary.wiley.com/doi/10.1111/zoj.12407/abstract
z| Artículo electrónico
901 _ _ a| Artículo con arbitraje
902 _ _ a| GOG / MM
904 _ _ a| Noviembre 2016
905 _ _ a| Artecosur
905 _ _ a| Biblioelectrónica
LNG eng
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Uncovering species boundaries in the Neotropical ant complex Ectatomma ruidum (Ectatomminae) under the presence of nuclear mitochondrial paralogues
Aguilar Velasco, Reina Gabriela (autor)
Poteaux, Chantal (autor)
Meza Lázaro, Rubí (autor)
Lachaud, Jean Paul (autor)
Dubovikoff, Dmitry A. (autor)
Zaldívar Riverón, Alejandro (autor)
Contenido en: Zoological Journal of the Linnean Society. Vol. 178, no. 2 (October 2016), p. 226–240. ISSN: 1096-3642
No. de sistema: 9804
Tipo: - Artículo con arbitraje
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Inglés

"Nuclear mitochondrial (mt) paralogues (numts) are non-functional fragments of mtDNA integrated into the nuclear genome that can overestimate the number of species in analyses based on mtDNA sequences. As numts have relatively slow mutation rates, they can pass undetected by conventional procedures such as inspecting for internal stop codons, indels or apparent polymorphism in chromatograms. Species boundaries based on mtDNA markers therefore require a thorough assessment of numts, especially in insects, where this phenomenon appears to be relatively frequent. Ectatomma ruidum is a widely distributed Neotropical ant species that is distributed from northern Mexico to northern Brazil. Previous behavioural and molecular evidence suggests that this species actually represents a composite taxon. Here we assessed the species boundaries in E. ruidum based on two mt (COI, cyt b) and one nuclear (H3) marker, as well as on external morphology. Ancient and recent mt paralogues were detected in several specimens, although pre-PCR dilution of DNA template helped to recover most of the mt orthologues. Based on the congruence found between our species delineation obtained from the mt genealogies and the discriminated morphospecies, we propose that E. ruidum is actually composed of at least three species. Two of these species have a wide geographical distribution in the Neotropics, whereas the remaining one was restricted to localities situated near the Pacific coast in south-east Mexico. We also found extensive intra- and interspecific variation in the barcoding locus. Moreover, the nuclear evidence suggests the existence of hybrids between two of these species in Oaxaca, south-east Mexico. This study agrees with previous studies of other closely related animal taxa, which have revealed a complex evolutionary history and overlooked species diversity in the latter region."