Cerrar

No. de sistema: 000037362

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 130701m20139999xx^^r^p^^^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
044 _ _ a| xx
100 1 _ a| Prosser, Sean
245 1 2 a| A new set of primers for COI amplification from freshwater microcrustaceans
520 1 _ a| Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of COI amplification has been quite variable when it comes to freshwater zooplankton (El ıas-Guti errez & Valdez-Moreno 2008; Jeffery etal. 2011). Some genera of microcrustaceans seem to be more difficult to amplify than others. For example, Macrothrix, Scapholeberis, Diaphanosoma and cyclopoids have yielded limited results. Among several possible reasons for the inability to barcode freshwater microcrustaceans is that there does not exist a specific set of primers for COI amplification. To this end, we developed a zooplankton – specific set of primers, which signifi- cantly increased average amplification success (20% increase). With these primers, we observed an overall success of over 70% for Sididae and Chydoridae, and more than 80% for Daphniidae, Moinidae, Bosminidae, Macrothricidae, Ilyocryptidae and Diaptomidae. We also demonstrate a simple alteration to a common specimen fixation method that increases the overall recovery of barcodes from freshwater zooplankton. Collectively, we believe our results will greatly aid the recovery of barcodes from these difficult groups.
650 _ 4 a| Microcrustáceos de agua dulce
650 _ 4 a| Copépodos
650 _ 4 a| Cladóceros
650 _ 4 a| Códigos de barras de ADN
700 1 _ a| Martínez Arce, Arely
e| coaut.
n| 55882142800
700 1 _ a| Elías Gutiérrez, Manuel
e| coaut.
773 0 _
t| Molecular Ecology Resources
g| Vol. 13, no. 6 (2013), p. 1151–1155
x| 1755-0998
901 _ _ a| Artículo con arbitraje
902 _ _ a| GOG / MM
904 _ _ a| Julio 2013
905 _ _ a| Artecosur
905 _ _ a| Biblioelectrónica
LNG eng
Cerrar
A new set of primers for COI amplification from freshwater microcrustaceans
Prosser, Sean (autor)
Martínez Arce, Arely (autor)
Elías Gutiérrez, Manuel (autor)
Contenido en: Molecular Ecology Resources. Vol. 13, no. 6 (2013), p. 1151–1155. ISSN: 1755-0998
No. de sistema: 37362
Tipo: - Artículo con arbitraje
  • Consulta (1)




Inglés

"Despite the contribution of DNA barcoding towards understanding the biodiversity and distribution of species, the success of COI amplification has been quite variable when it comes to freshwater zooplankton (El ıas-Guti errez & Valdez-Moreno 2008; Jeffery etal. 2011). Some genera of microcrustaceans seem to be more difficult to amplify than others. For example, Macrothrix, Scapholeberis, Diaphanosoma and cyclopoids have yielded limited results. Among several possible reasons for the inability to barcode freshwater microcrustaceans is that there does not exist a specific set of primers for COI amplification. To this end, we developed a zooplankton – specific set of primers, which signifi- cantly increased average amplification success (20% increase). With these primers, we observed an overall success of over 70% for Sididae and Chydoridae, and more than 80% for Daphniidae, Moinidae, Bosminidae, Macrothricidae, Ilyocryptidae and Diaptomidae. We also demonstrate a simple alteration to a common specimen fixation method that increases the overall recovery of barcodes from freshwater zooplankton. Collectively, we believe our results will greatly aid the recovery of barcodes from these difficult groups."