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No. de sistema: 000058383

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 170809m20179999xx^^r^p^^^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
043 _ _ a| n-mx-cp
044 _ _ a| xx
245 0 0 a| Description of the sperm and spermatheca of Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) for the differentiation of mated and unmated females
506 _ _ a| Acceso electrónico sólo para usuarios de ECOSUR
520 1 _ a| The coffee berry borer, Hypothenemus hampei (Ferrari), is a major insect pest of coffee crops worldwide. As part of a program of studies aimed at efficient and sustainable pest control, a study was conducted to determine the mating status of adult females in the broader context of studying pest dispersal behavior. To that end, it was deemed important to determine the morphology of the H. hampei female reproductive tract. This study focused on direct observation of the spermatheca of unmated, and colonizing adult females obtained from natural field populations with a high probability of having mated, and the description of all organs of the reproductive tract using established standard staining and microscopy techniques. Spermathecae were extracted from the samples and sorted according to their origin (unmated or colonizing females). The morphology of sperm present in the female spermathecae was compared with that of sperm taken directly from male testes. Two staining techniques using Giemsa and chlorazol black allowed spermatheca and sperm to be distinguished from other tissues. Under these conditions, a more precise description of the spermatheca was achieved, including the spermathecal duct and spermathecal muscles, spermathecal gland, and, for the first time, sperm when present. These techniques permitted the accurate and rapid determination of the presence or absence of sperm in the spermathecae of H. hampei females, thereby establishing their mating status.
650 _ 4 a| Broca del cafeto
650 _ 4 a| Espermatozoides
650 _ 4 a| Espermateca
650 _ 4 a| Fertilidad
651 _ 4 a| Región Soconusco (Chiapas, México)
700 1 _ a| Román Ruíz, Ariana Karina
e| coaut.
n| 55195682000
700 1 _ a| Michel, Bruno
e| coaut.
700 1 _ a| Dufour, Bernard Pierre
e| coaut.
700 1 _ a| Rojas, Julio C.
e| coaut.
700 1 _ a| Cruz López, Leopoldo Caridad
c| Dr.
e| coaut.
700 1 _ a| Barrera, Juan F.
c| Doctor
e| coaut.
773 0 _
t| Annals of the Entomological Society of America
g| Vol. 110, no. 4 (July 2017), p. 353-359
x| 0013-8746
900 _ _ a| Solicítelo con su bibliotecario/a
901 _ _ a| Artículo con arbitraje
902 _ _ a| BG / MM
904 _ _ a| Agosto 2017
905 _ _ a| Artecosur
905 _ _ a| Artfrosur
905 _ _ a| Café
905 _ _ a| Biblioelectrónica
906 _ _ a| Producción Académica ECOSUR
LNG eng
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*Solicítelo con su bibliotecario/a
Description of the sperm and spermatheca of Hypothenemus hampei (Coleoptera: Curculionidae: Scolytinae) for the differentiation of mated and unmated females
Román Ruíz, Ariana Karina (autor)
Michel, Bruno (autor)
Dufour, Bernard Pierre (autor)
Rojas, Julio C. (autor)
Cruz López, Leopoldo Caridad (autor)
Barrera, Juan F. (autor)
Nota: Acceso electrónico sólo para usuarios de ECOSUR
Contenido en: Annals of the Entomological Society of America. Vol. 110, no. 4 (July 2017), p. 353-359. ISSN: 0013-8746
No. de sistema: 58383
Tipo: - Artículo con arbitraje
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Inglés

"The coffee berry borer, Hypothenemus hampei (Ferrari), is a major insect pest of coffee crops worldwide. As part of a program of studies aimed at efficient and sustainable pest control, a study was conducted to determine the mating status of adult females in the broader context of studying pest dispersal behavior. To that end, it was deemed important to determine the morphology of the H. hampei female reproductive tract. This study focused on direct observation of the spermatheca of unmated, and colonizing adult females obtained from natural field populations with a high probability of having mated, and the description of all organs of the reproductive tract using established standard staining and microscopy techniques. Spermathecae were extracted from the samples and sorted according to their origin (unmated or colonizing females). The morphology of sperm present in the female spermathecae was compared with that of sperm taken directly from male testes. Two staining techniques using Giemsa and chlorazol black allowed spermatheca and sperm to be distinguished from other tissues. Under these conditions, a more precise description of the spermatheca was achieved, including the spermathecal duct and spermathecal muscles, spermathecal gland, and, for the first time, sperm when present. These techniques permitted the accurate and rapid determination of the presence or absence of sperm in the spermathecae of H. hampei females, thereby establishing their mating status."