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No. de sistema: 000059271

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 181218m20189999xx^^r^p^^^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
044 _ _ a| xx
245 0 0 a| Magnetic nanoparticle-based biosensing assay quantitatively enhances acid-fast bacilli count in paucibacillary pulmonary tuberculosis
520 1 _ a| A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+” (in SSM) to “2+”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1+”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs.
533 _ _ a| Reproducción electrónica en formato PDF
538 _ _ a| Adobe Acrobat profesional 6.0 o superior
650 _ 4 a| Mycobacterium tuberculosis
650 _ 4 a| Tuberculosis pulmonar
650 _ 4 a| Nanotecnología
650 _ 4 a| Enfermedades transmisibles
700 1 _ a| Gordillo Marroquín, Cristina
700 1 _ a| Gómez Velasco, Anaximandro
e| coaut.
700 1 _ a| Sánchez Pérez, Héctor Javier
d| 1960-
e| coaut.
700 1 _ a| Pryg, Kasey
e| coaut.
700 1 _ a| Shinners, John
e| coaut.
700 1 _ a| Murray, Nathan
e| coaut.
700 1 _ a| Muñoz Jiménez, Sergio G.
e| coaut.
700 1 _ a| Bencomo Alerm, Allied
e| coaut.
700 1 _ a| Gómez Bustamante, Adriana
e| coaut.
700 1 _ a| Jonapá Gómez, Letisia
e| coaut.
700 1 _ a| Enríquez Ríos, Natán
e| coaut.
700 1 _ a| Martín, Miguel
e| coaut.
700 1 _ a| Romero Sandoval, Natalia Cristina
c| Doctora
e| coaut.
700 1 _ a| Alocilja, Evangelyn C.
e| coaut.
773 0 _
t| Biosensors
g| Vol. 8, no. 4, 128 (December 2018), p. 1-13
x| 2079-6374
856 4 1 u| https://www.mdpi.com/2079-6374/8/4/128
z| Artículo electrónico
856 _ _ u| http://aleph.ecosur.mx:8991/F?func=service&doc_library=CFS01&local_base=CFS01&doc_number=000059271&line_number=0001&func_code=DB_RECORDS&service_type=MEDIA
y| Artículo electrónico
901 _ _ a| Artículo con arbitraje
902 _ _ a| GOG
904 _ _ a| Diciembre 2018
905 _ _ a| Artecosur
905 _ _ a| Biblioelectrónica
906 _ _ a| Producción Académica ECOSUR
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"A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+” (in SSM) to “2+”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1+”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs."


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