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No. de sistema: 000059325

LDR _ _ 00000nab^^22^^^^^za^4500
008 _ _ 190115m20179999xx^^r^p^o^^^^z0^^^a0eng^d
040 _ _ a| ECO
c| ECO
044 _ _ a| xx
245 0 0 a| Protective effect of Lactobacillus casei on DMH-induced colon carcinogenesis in mice
506 _ _ a| Disponible para usuarios de ECOSUR con su clave de acceso
520 1 _ a| The administration of probiotics is a promising approach to reduce the prevalence of colon cancer, a multifactorial disease, with hereditary factors, as well as environmental lifestyle-related risk factors. Biogenic polyamines, putrescine, spermidine, and spermine are small cationic molecules with great roles in cell proliferation and differentiation as well as regulation of gene expression. Ornithine decarboxylase is the first rate-limiting enzyme for polyamine synthesis, and upregulation of ornithine decarboxylase activity and polyamine metabolism has been associated with abnormal cell proliferation. This paper is focused on studying the protective role of Lactobacillus casei ATCC 393 in a chemically induced mouse model of colon carcinogenesis, directing our attention on aberrant crypt foci as preneoplastic markers, and on polyamine metabolism as a possible key player in carcinogenesis. BALB/c mice were administered 1,2-dimethylhydrazine dihydrochloride (DMH) to induce colon cancer (20 mg/kg body weight, subcutaneous, twice a week for 24 weeks). L. casei ATCC 393 was given orally (10 6 CFU, twice a week), 2 weeks before DMH administration. Hematoxylin and eosin staining, high-performance liquid chromatography, and Western blotting were used to evaluate aberrant crypt foci, urinary polyamines, and ornithine decarboxylase expression in the colon. The experimental data showed that the preventive administration of L. casei ATCC 393 may delay the onset of cancer as it significantly reduced the number of DMH-induced aberrant crypt foci, the levels of putrescine, and the expression of ornithine decarboxylase. Hence, this probiotic strain has a prospective role in protection against colon carcinogenesis, and its antimutagenic activity may be associated with the maintenance of polyamine metabolism.
530 _ _ a| Disponible en línea
533 _ _ a| Reproducción electrónica en formato PDF
538 _ _ a| Adobe Acrobat profesional 6.0 o superior
650 _ 4 a| Lactobacillus casei
650 _ 4 a| Probióticos
650 _ 4 a| Carcinogénesis de cólon
650 _ 4 a| Ornitina descarboxilasa
700 1 _ a| Irecta Nájera, César Antonio
c| Doctor
e| autor
700 1 _ a| Huizar López, María del Rosario
e| autora
700 1 _ a| Casas Solís, Josefina
e| autora
700 1 _ a| Castro Félix, Patricia
e| autora
700 1 _ a| Santerre, Anne
e| autora
773 0 _
t| Probiotics and Antimicrobial Proteins
g| Vol. 9, no. 2 (June 2017), p. 163–171
x| 1867-1314
900 _ _ a| Solicítelo con su bibliotecario/a
901 _ _ a| Artículo con arbitraje
902 _ _ a| BG / MM
904 _ _ a| Enero 2019
905 _ _ a| Artecosur
905 _ _ a| Biblioelectrónica
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*Solicítelo con su bibliotecario/a
Protective effect of Lactobacillus casei on DMH-induced colon carcinogenesis in mice
Irecta Nájera, César Antonio (autor)
Huizar López, María del Rosario (autora)
Casas Solís, Josefina (autora)
Castro Félix, Patricia (autora)
Santerre, Anne (autora)
Nota: Disponible en línea
Disponible para usuarios de ECOSUR con su clave de acceso
Contenido en: Probiotics and Antimicrobial Proteins. Vol. 9, no. 2 (June 2017), p. 163–171. ISSN: 1867-1314
No. de sistema: 59325
Tipo: - Artículo con arbitraje
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"The administration of probiotics is a promising approach to reduce the prevalence of colon cancer, a multifactorial disease, with hereditary factors, as well as environmental lifestyle-related risk factors. Biogenic polyamines, putrescine, spermidine, and spermine are small cationic molecules with great roles in cell proliferation and differentiation as well as regulation of gene expression. Ornithine decarboxylase is the first rate-limiting enzyme for polyamine synthesis, and upregulation of ornithine decarboxylase activity and polyamine metabolism has been associated with abnormal cell proliferation. This paper is focused on studying the protective role of Lactobacillus casei ATCC 393 in a chemically induced mouse model of colon carcinogenesis, directing our attention on aberrant crypt foci as preneoplastic markers, and on polyamine metabolism as a possible key player in carcinogenesis. BALB/c mice were administered 1,2-dimethylhydrazine dihydrochloride (DMH) to induce colon cancer (20 mg/kg body weight, subcutaneous, twice a week for 24 weeks). L. casei ATCC 393 was given orally (10 6 CFU, twice a week), 2 weeks before DMH administration. Hematoxylin and eosin staining, high-performance liquid chromatography, and Western blotting were used to evaluate aberrant crypt foci, urinary polyamines, and ornithine decarboxylase expression in the colon. The experimental data showed that the preventive administration of L. casei ATCC 393 may delay the onset of cancer as it significantly reduced the number of DMH-induced aberrant crypt foci, the levels of putrescine, and the expression of ornithine decarboxylase. Hence, this probiotic strain has a prospective role in protection against colon carcinogenesis, and its antimutagenic activity may be associated with the maintenance of polyamine metabolism."


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