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136 resultados encontrados para: AUTOR: Elías Gutiérrez, Manuel
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1.
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Resumen en: Español |
Resumen en español

...de tierras abajo vengo, cantan los Huaches, Cochos, Chinos y uno que otro gachupin en jacarandoso fandango. Este disco es el inicio de una serie que busca guardar en cofre digital las joyas que la tradición ha pulido a fuerza de repetir el canto y ejercitar los dedos; pero sobretodo, queremos animar a los guachitos y guachitas a tomar los instrumentos de los abuelos para tocar la vieja y alegre música antes de que desaparezca en el polvo del olvido


2.
Artículo
A new species of Scapholeberis Schoedler, 1858 (Anomopoda: Daphniidae: Scapholeberinae) from the Colombian Amazon basin highlighted by DNA barcodes and morphology
Andrade Sossa, Camilo (autor) ; Buitron Caicedo, Lorena (autora) ; Elías Gutiérrez, Manuel (autor) ;
Disponible en línea
Contenido en: PeerJ Volumen 8, artículo número e9989 (2020), páginas 1-20 ISSN: 2167-8359
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Resumen en: Inglés |
Resumen en inglés

Background: The Amazon basin is recognized as one of the most complex and species-rich freshwater environments globally. The diversity of zooplankton here remains unknown, with many species undescribed. Methods: Here, we describe a new species of Scapholeberis Schoedler, 1858 (Cladocera: Anomopoda: Daphniidae: Scapholeberinae) from the Colombian Amazon Basin, collected with recently designed light traps. The description is based on detailed morphology (based on SEM and light microscopy) of parthenogenetic females, ephippial females, males, and molecular data based on the COI gene. Results: Scapholeberis yahuarcaquensis n. sp. has a combination of characters present in Scapholeberis kingi Sars, 1888 and Scapholeberis armata freyi Dumont & Pensaert, 1983. These are a trilobate rostrum, with the middle lobe well developed with sides straight to relatively rounded, the presence of an elongated slit frontal head pore, a dorsal pore in the juncture of the cephalic shield and the valves, and a single denticulate membrane at the posterior rim of the valves, with stronger setae in the last third. The unique characters of the parthenogenetic females are ventral sucker with delicate triangles.

Each has a filament-like projection in the lamellae’s inner side and an external section forming convex folds with denticle-like projections in the middle zone of the sucker-plate. There is a peculiar pitted sculpture in the ephippial females and a strong projection in the front of it. The male hook on the limb I with a blunt tip, a quirky lamella-like outgrow in the proximal side, and a paddle with well-developed spines scattered on its surface. The ventral sucker-lamellae in the male is much more developed than the female. The COI gene sequences showed an interspecific mean genetic divergence of 16.4% between S. yahuarcaquensis n. sp. and the closest species S. freyi from Mexico, supporting our results. A coalescence analysis and Barcode Index Number also support the new species based on the DNA sequences. New methods of collecting and integrative biology will give important support to recognize the fauna from the Amazon Basin, one of the most important sources of fresh water in the world that remains unknown in many respects.


3.
Artículo
Water mite diversity (Acariformes: Prostigmata: Parasitengonina: Hydrachnidiae) from karst ecosystems in southern of Mexico: a barcoding approach
Montes Ortiz, Lucia (autora) ; Elías Gutiérrez, Manuel (autor) ;
Disponible en línea
Contenido en: Diversity Volumen 12, número 9, 329 (August 2020), p. 1-16 ISSN: 1424-2818
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Resumen en: Inglés |
Resumen en inglés

Water mites represent the most diverse and abundant group of Arachnida in freshwater ecosystems, with about 6000 species described; however, it is estimated that this number represents only 30% of the total expected species. Despite having strong biotic interactions with their community and having the potential to be exceptional bioindicators, they are frequently excluded from studies of water quality or ecology, due to actual and perceived difficulties of taxonomic identification in this group. The objective of this study is to use the variations in the sequences of the mitochondrial cytochrome oxidase subunit I (COI), also known as the DNA barcodes region, as a tool to assess the diversity of water mites at 24 sites in the Yucatan Peninsula of Mexico. We found 77 genetic groups or putative species corresponding to 18 genera: Arrenurus, Atractides, Centrolimnesia, Eylais, Geayia, Hydrodroma, Hydryphantes, Hygrobates, Koenikea, Krendowskia, Limnesia, Limnochares, Mamersellides, Mideopsis, Neumania, Piona, Torrenticola, and Unionicola. This was significant, since there are only 35 species described for this region. Furthermore, this molecular information has allowed us to infer that there are characteristic assemblies per site. These data will facilitate the incorporation of water mites in different studies while the curatorial work continues to assign a Linnaean name.


4.
Artículo
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Resumen en: Inglés |
Resumen en inglés

The Brachionus plicatilis complex represents the most studied group of rotifers, although the systematics of the species complex has not been completely clarified. Many studies have been conducted trying to explore the diversity within the complex, leading to the recognition of three major morphotypes: large (L), small-medium (SM), and small (SS). Currently six species have been described and classified under these types and another nine taxa have been identified but not formally described. Within the L group, three species have been officially described [B. plicatilis s.s. (L1), B. manjavacas (L2), and B. asplanchnoidis (L3)], while a formal description of L4, unofficially known as B. ‘Nevada’, is still lacking. In the present study, a new species, Brachionus paranguensis sp. nov., is formally described and presented as a repre ZooKeys sentative of the L4 clade. The species has been named after a high altitude saline crater lake from Central Mexico, where the specimens were collected. An integrated approach using DNA taxonomy through COI and ITS1 markers, morphology, and ecology was used to confirm the identity of the new species.


5.
- Artículo con arbitraje
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DNA barcoding as a diagnostic tool of a rare human parasitosis: the first case of Lagochilascaris minor in Quintana Roo, Mexico
González Solís, David ; Elías Gutiérrez, Manuel (coaut.) ; Prado Bernal, Jenny Alexandra (coaut.) ; García de la Cruz, Miguel Alfredo (coaut.) ;
Contenido en: Journal of Parasitology Vol. 105, no. 2 (2019), p. 351–358 ISSN: 0022-3395
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Resumen en: Inglés |
Resumen en inglés

Recently, DNA barcoding based on the mitochondrial gene cytochrome c oxidase subunit 1 (COI) has become a widespread tool to identify animals. Its use with parasites of humans has been limited with some groups of nematodes where the amplification of this gene has been difficult. In this study, we present the first COI barcode sequence of a rare parasite from tropical regions, Lagochilascaris minor, which parasitized a human host from Quintana Roo, southern Yucatán Peninsula, Mexico. Destruction of the mastoid apophysis in the lateral sinus and cerebellar involvement were observed at the site of infection. After a radical mastoidectomy and a treatment with 200 mg oral albendazole for 63 days, the patient completely recovered. Lagochilascaris minor was identified based on the ratio between length of spicules and ejaculatory duct, shape of eggs, and host, as well as comparison with its congeners. The mode of infection is unknown, although it could be after direct exposure to eggs or consumption of uncooked wild meat. Morphology of adults is demonstrated using scanning electron microscopy, and high-quality sequences of COI barcode are presented from amplifications using semi-degenerate primers designed for micro-crustaceans. DNA barcoding proved to be a reliable identification method for L. minor. A comparison of the sequences for this species with 81 ascaridoids obtained from the Barcode of Life Database places it in a unique clade most closely related to Baylisascaris procyonis. Future diagnosis of larval and adult stages of L. minor using DNA barcoding will allow the recognition of its infection parameters, transmission, and precise epidemiology. Reports of lagochilascarosis in the Yucata´n Peninsula have been occurred over the last decade, suggesting it is an emerging zoonotic disease in the region.


6.
- Artículo con arbitraje
Faunistic survey of the zooplankton community in an oligotrophic sinkhole, Cenote Azul (Quintana Roo, Mexico), using different sampling methods, and documented with DNA barcodes
Montes Ortiz, Lucia (autora) ; Elías Gutiérrez, Manuel (autor) ;
Disponible en línea
Contenido en: Journal of Limnology Vol. 77, no. 3 (Jun 2018 ), p. 428-440 ISSN: 1129-5767
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Resumen en: Inglés |
Resumen en inglés

This study is the first faunistic inventory of a zooplankton community from an open, karstic and oligotrophic aquatic sinkhole in the south of the Yucatan Peninsula (Mexico), we describe the richness of the zooplankton collected with the combination of plankton nets and light traps of our own design, using morphological and molecular characters to identify the species and demonstrate the effectiveness of only one set of primers to sequence all taxa. Recently, it has been demonstrated that different sampling methods can increase the number of zooplankton species from tropical and temperate systems dramatically. These more effective methods together with DNA barcoding can give a new and more realistic picture of the species dwelling in a freshwater system. In total, we sequenced 268 specimens, and the list of species known in this sinkhole increased from 13 to 77 taxa, with a projection of 87 in total, including cladocerans, copepods, ostracods, fish larvae, tadpoles, rotifers, chironomids, water mites, among others. From the 77 taxa identified by us, 72 BINS (Barcode Index Numbers, equivalent to putative species) were assigned by the BOLD Database (boldsystems.org), and 30 of them are new records for both, BOLD and GenBank (www.ncbi.nlm.nih.gov). There was an essential difference in the number of taxa collected with the plankton nets and the light traps. Only 23 taxa were registered in the nets, representing between 28 and 29% of the total richness, while 67 were present in the light traps representing 87% of the species found. From these, 46 taxa were exclusive to this sampling method. Light traps are an effective method for rapid evaluation of zooplankton.

In the future, combining DNA barcoding and high-throughput sequencing methods with more efficient collecting will enable us to perform quick and precise biomonitoring of any aquatic ecosystem, enabling the detection of changes in zooplankton composition resulting from climate change and anthropogenic disturbances. Nevertheless, as a first step it is fundamental to establish a baseline of DNA barcodes for the species in these ecosystems.


7.
- Artículo con arbitraje
First evidence of parasitation of a Bosmina (Cladocera) by a water mite larva in a karst sinkhole, in Quintana Roo (Yucatán Peninsula, México)
Montes Ortiz, Lucia ; Goldschmidt, Tom (coaut.) ; Elías Gutiérrez, Manuel (coaut.) ;
Contenido en: Acarologia Vol. 59, no. 1 (2019), p. 111-114 ISSN: 0044-586X
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Resumen en: Inglés |
Resumen en inglés

For the first time a parasitic relationship between a water mite larva and a Cladocera is found and documented by scanning electron microscope (SEM) imaging. A Unionicolidaelarva (cf. Unionicola) has been found attached to aBosmina tubicen (Cladocera) collected in a karst sinkhole (cenote) in the southeast of the Yucatán Peninsula (México).


8.
- Artículo con arbitraje
Moina macrocopa (Straus, 1820): a species complex of a common cladocera, highlighted by morphology and DNA barcodes
Montoliu Elena, Lucía ; Elías Gutiérrez, Manuel (coaut.) ; Silva Briano, Marcelo (coaut.) ;
Contenido en: Limnetica Vol. 38, no. 1 (January 2019), p. 253-277 ISSN: 0213-8409
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Resumen en: Español | Inglés |
Resumen en español

Los microcrustáceos de aguas continentales son uno de los grupos más diverso y menos estudiado del reino animal. Un enfoque integrativo, que incluya al menos, datos morfológicos, moleculares y geográficos es esencial para delimitar las especies de estos invertebrados, como es el caso del complejo Moina macrocopa. En este estudio, utilizamos tres tipos de caracteres: morfológicos, genéticos (códigos de barras del ADN) y geográficos, para demostrar que Moina macrocopa(Straus, 1820), el clado del Viejo Mundo, considerado aquí como M. macrocopa s.l., y Moina macrocopa americana Goulden, 1968, el clado americano, son especies distintas, no subespecies. Además, confirmamos que Moina macrocopa s.l. es un complejo de especies, formado por un mínimo de 3 clados diferentes.

Resumen en inglés

Freshwater microcrustaceans are one of the most diverse and understudied groups of the animal kingdom and are best described by the use of an integrative taxonomic approach, including morphological, molecular and geographical data. In this study, we used three different types of characters, including detailed morphological analyses, DNA barcodes (COI gene) and geographi-cal distribution to study the Old-World clade Moina macrocopa (Straus, 1820), here considered as M. macrocopa s.l. and the American clade Moina macrocopa americana Goulden, 1968. Results have indicated that these clades are different species and not subspecies, while Moina macrocopa s.l. is suggested to be species complex with a minimum of three different clades.


9.
Artículo
Using eDNA to biomonitor the fish community in a tropical oligotrophic lake
Valdéz Moreno, Martha (autora) ; Ivanova, Natalia V. (autora) ; Elías Gutiérrez, Manuel (autor) ; Pedersen, Stephanie L. (autora) ; Bessonov, Kyrylo (autor) ; Hebert, Paul D. N. (autor) ;
Contenido en: PLoS One Vol. 14, no. 4, e0215505 (April 2019), p. 1-22 ISSN: 0173-9565
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Resumen en: Inglés |
Resumen en inglés

Environmental DNA (eDNA) is an effective approach for detecting vertebrates and plants, especially in aquatic ecosystems, but prior studies have largely examined eDNA in cool temperate settings. By contrast, this study employs eDNA to survey the fish fauna in tropical Lake Bacalar (Mexico) with the additional goal of assessing the possible presence of invasive fishes, such as Amazon sailfin catfish and tilapia. Sediment and water samples were collected from eight stations in Lake Bacalar on three occasions over a 4-month interval. Each sample was stored in the presence or absence of lysis buffer to compare eDNA recovery. Short fragments (184–187 bp) of the cytochrome c oxidase I (COI) gene were amplified using fusion primers and then sequenced on Ion Torrent PGM or S5 before their source species were determined using a custom reference sequence database constructed on BOLD. In total, eDNA sequences were recovered from 75 species of vertebrates including 47 fishes, 15 birds, 7 mammals, 5 reptiles, and 1 amphibian. Although all species are known from this region, six fish species represent new records for the study area, while two require verification. Sequences for five species (2 birds, 2 mammals, 1 reptile) were only detected from sediments, while sequences from 52 species were only recovered from water. Because DNA from the Amazon sailfin catfish was not detected, we used a mock eDNA experiment to confirm our methods would enable its detection. In summary, we developed protocols that recovered eDNA from tropical oligotrophic aquatic ecosystems and confirmed their effectiveness in detecting fishes and diverse species of vertebrates.


10.
- Artículo con arbitraje
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Resumen en: Español | Inglés |
Resumen en español

Moina micrura Kurz, 1875 (Anomopoda: Moinidae) es una de las especies de cladóceros más confusas del mundo. Durante mucho tiempo fue considerada cosmopolita pero análisis moleculares recientes corroboraron la idea de que es un complejo de especies crípticas estrechamente relacionadas. Los persistentes problemas sistemáticos con M. micrura derivan de la falta de una redescripción detallada y de que tanto su material tipo como la localidad tipo han desaparecido. La importancia de esta especie radica en que es utilizada en diversos experimentos de índole ecotoxicológica, fisiológica, como alimento vivo, etc. Con este estudio, tratamos de proporcionar una redescripción de M. micrura s. str, utilizando la morfología de las hembras partenogenéticas, efipiales y de los machos, así como caracteres adicionales como los códigos de barras de ADN. El material estudiado procede de un estanque situado cerca de la localidad tipo original en la República Checa. En primer lugar, se secuenciaron los genes mitocondriales citocromo c oxidasa subunidad I (COI o códigos de barras) y el 12S a fin de establecer la identidad de M. micrura s. str. Después de este análisis, se compararon las secuencias obtenidas con todas las secuencias publicadas disponibles de esta especie a nivel mundial. Aparentemente, M. micrura s. str. está restringida al Paleártico, con registros confirmados molecularmente en República Checa, Eslovaquia, Kazajstán e Israel.

El linaje más próximo a M. micrura s. str. se encontró en España pero se necesita un análisis más exhaustivo para establecer con certeza su identidad. M. micrura s. str. presenta características únicas como la espinulación posterior de las valvas de la hembra, y la ornamentación del efipio. En el macho, las espínulas en la punta de la antena y el gancho del primer toracópodo también son únicos. Con esta descripción, esperamos que los especialistas tengan una referencia clara para descubrir la diversidad de este complejo, así como comprender su biogeografía y diversidad.

Resumen en inglés

Moina micrura Kurz, 1875 (Anomopoda: Moinidae) belongs among the most poorly defined cladoceran species in the world. This species has been considered cosmopolitan and is widely used for laboratory experiments, ecotoxicology, physiology or as live food. Nevertheless, recent molecular analyses corroborated the idea that it is a diverse complex of closely related species. Persisting systematic problems with M. micrura stem from the fact that it has not been redescribed in detail, and its type material as well as the type locality are lost. With this study, we try to provide a redescription, using morphology of females, males and ephippial females and the DNA barcodes for M. micrura s. str. from the pond situated not far from its original type locality in the Czech Republic. Firstly, we sequenced mitochondrial genes for cytochrome c oxidase subunit I (COI) and the 12S rDNA to establish the identity of M. micrura s. str. After this analysis, we compared the genetic data with all available sequences across the world. The species seems restricted to the Western Palearctic, with the most easterly located genetically confirmed records from Kazakhstan and Israel. The closest related lineage to M. micrura s. str. was found in Spain and needs a thorough analysis to establish its systematic status. M. micrura s. str. has unique features in the posterior spinulation of the valves of the parthenogenetic female, and the ornamentation of the ephippium. In the male, spinules on the tip of the antennule and the hook on the first thoracopod are also unique. With this description, we hope to inspire specialists to start uncovering the diversity of this complex, to understand its biogeography and diversity, as well as the real range of M. micrura s. str.