Términos relacionados

2 resultados encontrados para: AUTOR: Gómez Bustamante, Adriana
  • «
  • 1 de 1
  • »
1.
Artículo
Resumen en español

Las enfermedades infecciosas (EIs) emergentes, re-emergentes y las tropicales desatendidas pueden ser localizadas en áreas geográficas específicas, pero fenómenos como el cambio climático, la globalización, las condiciones socioeconómicas y la migración, entre otros, pueden facilitar su rápida transmisión hacia otras regiones. Brotes recientes de Zika, Chikungunya, y Ébola han puesto en alerta la salud pública mundial. Otras enfermedades infecciosas aún persisten y representan severos problemas de salud pública, tales como la tuberculosis, el VIH-sida, la leishmaniasis, el dengue, la malaria y la enfermedad de Chagas, entre otras. Para el diagnóstico, prevención y disminución en la propagación de las EIs, es necesario implementar métodos de diagnóstico precisos, rápidos y accesibles que permitan su atención médica de manera oportuna. Describimos el uso de la nanotecnología, específicamente de “Alocilja Nanopartículas Magnéticas (AMNs)” acopladas a un biosensor, como un nuevo método de diagnóstico de las EIs. Ilustramos su uso potencial para el diagnóstico de la tuberculosis.

Resumen en inglés

Emerging, re-emerging and neglected tropical infectious diseases (IDs) can be restricted to specific geographic zones, but climate change, globalization, social conditions, and migration, among other factors, can trigger their dissemination worldwide. Outbreaks of Zika, Chikungunya and Ebola have threatened public health worldwide. The rapid transmission of these viral diseases exemplifies the potential quick spread of an infectious disease. Yet several infectious diseases that are of major concern for public health, namely tuberculosis, VIH-sida, Leishmaniasis, dengue, malaria, Chagas, among others. To detect and combat the spread of these IDs, we urge, rapid, and accessible diagnostic tests are needed to identify specific pathogens in order to provide early medical care. In this paper, we review the use of nanotechnology, specifically the Alocilja functionalized magnetic nanoparticles (AMNs) coupled to a biosensor, as a new and quick diagnostic test to detect IDs, and exemplify their potential use in the diagnosis of tuberculosis.


PDF PDF
Resumen en: Inglés |
Resumen en inglés

A new method using a magnetic nanoparticle-based colorimetric biosensing assay (NCBA) was compared with sputum smear microscopy (SSM) for the detection of pulmonary tuberculosis (PTB) in sputum samples. Studies were made to compare the NCBA against SSM using sputum samples collected from PTB patients prior to receiving treatment. Experiments were also conducted to determine the appropriate concentration of glycan-functionalized magnetic nanoparticles (GMNP) used in the NCBA and to evaluate the optimal digestion/decontamination solution to increase the extraction, concentration and detection of acid-fast bacilli (AFB). The optimized NCBA consisted of a 1:1 mixture of 0.4% NaOH and 4% N-acetyl-L-cysteine (NALC) to homogenize the sputum sample. Additionally, 10 mg/mL of GMNP was added to isolate and concentrate the AFB. All TB positive sputum samples were identified with an increased AFB count of 47% compared to SSM, demonstrating GMNP’s ability to extract and concentrate AFB. Results showed that NCBA increased AFB count compared to SSM, improving the grade from “1+” (in SSM) to “2+”. Extending the finding to paucibacillary cases, there is the likelihood of a “scant” grade to become “1+”. The assay uses a simple magnet and only costs $0.10/test. NCBA has great potential application in TB control programs.