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3 resultados encontrados para: AUTOR: Ivanova, Natalia V.
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Using eDNA to biomonitor the fish community in a tropical oligotrophic lake
Valdéz Moreno, Martha (autora) ; Ivanova, Natalia V. (autora) ; Elías Gutiérrez, Manuel (autor) ; Pedersen, Stephanie L. (autora) ; Bessonov, Kyrylo (autor) ; Hebert, Paul D. N. (autor) ;
Contenido en: PLoS One Vol. 14, no. 4, e0215505 (April 2019), p. 1-22 ISSN: 0173-9565
Resumen en: Inglés |
Resumen en inglés

Environmental DNA (eDNA) is an effective approach for detecting vertebrates and plants, especially in aquatic ecosystems, but prior studies have largely examined eDNA in cool temperate settings. By contrast, this study employs eDNA to survey the fish fauna in tropical Lake Bacalar (Mexico) with the additional goal of assessing the possible presence of invasive fishes, such as Amazon sailfin catfish and tilapia. Sediment and water samples were collected from eight stations in Lake Bacalar on three occasions over a 4-month interval. Each sample was stored in the presence or absence of lysis buffer to compare eDNA recovery. Short fragments (184–187 bp) of the cytochrome c oxidase I (COI) gene were amplified using fusion primers and then sequenced on Ion Torrent PGM or S5 before their source species were determined using a custom reference sequence database constructed on BOLD. In total, eDNA sequences were recovered from 75 species of vertebrates including 47 fishes, 15 birds, 7 mammals, 5 reptiles, and 1 amphibian. Although all species are known from this region, six fish species represent new records for the study area, while two require verification. Sequences for five species (2 birds, 2 mammals, 1 reptile) were only detected from sediments, while sequences from 52 species were only recovered from water. Because DNA from the Amazon sailfin catfish was not detected, we used a mock eDNA experiment to confirm our methods would enable its detection. In summary, we developed protocols that recovered eDNA from tropical oligotrophic aquatic ecosystems and confirmed their effectiveness in detecting fishes and diverse species of vertebrates.

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Using DNA barcodes to connect adults and early life stages of marine fishes from the Yucatan Peninsula, Mexico: potential in fisheries management
Valdéz Moreno, Martha ; Vásquez Yeomans, Lourdes (coaut.) ; Elías Gutiérrez, Manuel (coaut.) ; Ivanova, Natalia V. (coaut.) ; Herbert, Paul D. N. (coaut.) ;
Clasificación: AR/574.873282 / U9
Contenido en: Marine and Freshwater Research Vol. 61, no. 6 (June 2010), p. 665-671 ISSN: 1323-1650
Bibliotecas: Chetumal
SIBE Chetumal
ECO030007223 (Disponible)
Disponibles para prestamo: 1
Resumen en: Inglés |
Resumen en inglés

Barcoding has proven a useful tool in the rapid identification of all life stages of fish species. Such information is of critical importance for fisheries management and conservation, especially in high-diversity regions, such as Mexico’s marine waters, where more than 2200 species occur. The present study reports the barcode analysis of 1392 specimens from the Yucatan Peninsula, corresponding to 610 adults and juveniles, 757 larvae and 25 eggs, representing 181 species (179 teleosts and 2 rays), 136 genera and 74 families. Barcoding results revealed major range extensions and overlooked taxa, including three sympatric species of Albula (one likely undescribed) and a new taxon of Floridichthys. In total, six species of eggs and 34 species of larvae were identified through their barcode match with adults. These cases enabled the first discrimination of the larvae of four species of Eucinostomus, and new information about spawning locality and time was obtained from egg records for the hogfish, Lachnolaimus maximus, which is one of the most commercially important species in the Mexican Caribbean. Also, barcodes revealed mistakes in species recognition during a sport-fish contest. In the future, barcodes will help avoid similar errors and protect rare or endangered species, and will aid regulation of fisheries quotas.

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DNA barcodes for Cladocera and Copepoda from Mexico and Guatemala, highlights and new discoveries
Elías Gutiérrez, Manuel ; Martínez Jerónimo, Fernando (coaut.) ; Ivanova, Natalia V. (coaut.) ; Valdéz Moreno, Martha (coaut.) ; Hebert, Paul D. N. (coaut.) ;
Contenido en: Zootaxa No. 1839 (2008), p. 1–42 ISSN: 1175-5326
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

DNA barcoding, based on sequence diversity in the mitochondrial COI gene, has proven an excellent tool for identifying species in many animal groups. Here, we report the first barcode studies for freshwater zooplankton from Mexico and Guatemala and discuss the taxonomic and biological implications of this work. Our studies examined 61 species of Cladocera and 21 of Copepoda, about 40% of the known fauna in this region. Sequence divergences among conspecific individuals of cladocerans and copepods averaged 0.82% and 0.79%, respectively, while sequence divergences among congeneric taxa were on average 15-20 times as high. Barcodes were successful in discriminating all species in our study, but sequences for Mexican Daphnia exilis overlapped with those of D. spinulata from Argentina. Our barcode data revealed evidence of many species overlooked by current classification systems —for example, based on COI genotypes the Diapahanosoma birgei group appears to include 5 species, while Ceriodaphnia cf. rigaudi, Moina cf. micrura, Mastigodiaptomus albuquerquensis and Mastigodiaptomus reidae all include 2–3 taxa. The barcode results support recent taxonomic revisions, such as recognition of the genus Leberis, and the presence of several species in the D. birgei and Chydorus sphaericus complexes. The present results indicate that DNA barcoding will provide powerful new insights into both the incidence of cryptic species and a better understanding of zooplankton distributions, aiding evaluation of the factors influencing competitive outcomes, and the colonization of aquatic environments.