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11 resultados encontrados para: AUTOR: Rendón Arana, Pedro Alfonso
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1.
- Artículo con arbitraje
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Demography of a genetic sexing strain of Anastrepha ludens (Diptera: Tephritidae): effects of selection based on mating performance
Quintero Fong, José Luis ; Toledo, Jorge (coaut.) ; Ruiz Montoya, Lorena (coaut.) (1964-) ; Rendón Arana, Pedro Alfonso (coaut.) ; Orozco Dávila, Dina (coaut.) ; Valle Mora, Javier Francisco (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Agricultural and Forest Entomology Vol. 20, no. 1 (February 2018), p. 1-8 ISSN: 1461-9555
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Resumen en: Inglés |
Resumen en inglés

1. Tapachula-7 is a genetic sexing strain of Anastrepha ludens (Loew) (Diptera: Tephritidae) developed for the application of the sterile insect technique (SIT). To improve the sexual performance of this strain, a mass-reared colony was established from males selected for their sexual competitiveness. 2. Males from selected colonies are more sexually competitive than nonselected males. The present study aimed to analyse the demographic changes recorded in the study colony throughout four consecutive generations, comparing this colony with the parental colony and a wild strain. 3. The results obtained showed that, in the selected strain, fecundity increased, whereas survival diminished, compared with the laboratory parental strain. The increases in fecundity rates were observed at the first generation after selection. No changes were observed in the duration of the reproductive period in the selected strain. 4. Compared with wild flies, selected flies had lower life expectancy, earlier and shorter reproductive period, and greater daily fecundity at young ages (10–30 days), although with lower lifetime fecundity rates. 5. The four generations of the selected colony showed similar patterns of survival and reproduction. The better mating performance and the increase in early fecundity suggest that selection could contribute to improve rearing efficiency and SIT effectiveness.


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Resumen en: Inglés |
Resumen en inglés

The transgenic strain of the Mediterranean fruit fly (medfly), Ceratitis capitata (Wied.) VIENNA 8 1260, developed from the classical genetic sexing strain VIENNA 8, has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testicles and sperm. These traits offer a precise tool to discriminate between mass-reared sterile males and wild fertile males, and they could potentially increase the effectiveness of control programs for this pest. To assess the risk of horizontal transfer of the fluorescence transgenes in natural ecosystems, we used the VIENNA 8 1260 strain and the medfly parasitoid Fopius ceratitivorus. The fluorescence signal and the inheritance of the fluorescence gene markers were monitored for over 16 generations (about two years) in both species using fluorescence microscopy and a PCR-based assay. The PCR analysis was performed in four independent laboratories. Both fluorescence microscopy and PCR analysis indicated that no horizontal gene transfer of the DsRed transgene occurred during 16 generations of medfly parasitoid rearing under experimental conditions.


3.
Artículo
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Effect of irradiation doses on sterility and biological security in a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae)
Ramírez Santos, Edwin Mauricio ; Rendón Arana, Pedro Alfonso (coaut.) ; Ruiz Montoya, Lorena (coaut.) (1964-) ; Toledo, Jorge (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Journal of Economic Entomology Vol. 110, no. 4 (August 2017), p. 1483–1494 ISSN: 1938-291X
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Resumen en: Inglés |
Resumen en inglés

The genetically modified strain of the Mediterranean fruit fly, Ceratitis capitata (Wiedemann) VIENNA 8 1260, was developed from the genetic sexing strain VIENNA 8. It has two molecular markers that exhibit red fluorescence in the body and green fluorescence in testis and sperm. These traits offer a precise tool to discriminate between mass-reared and wild males, increasing the effectiveness of sterile insect technique. The reproductive performance of the VIENNA 8 1260 and VIENNA 8 D53- (without the D53 inversion introduced to prevent recombination) was compared at different irradiation doses. The general effect of irradiation on VIENNA 8 1260 followed the same patterns documented in previous publications for VIENNA 8 D53-. Irradiation doses of 80 Gray or greater reduced fertility and induced high levels of sterility in wild females. Fecundity reduction was higher in VIENNA 8 1260 than in VIENNA 8 D53- females. Vertical transmission of the fluorescence gene was confirmed up to the F4 generation. Substerilization in the VIENNA 8 1260 could jeopardize the usefulness of the transgenic strain due to the possible vertical transfer of the fluorescence transgene from the sterile males to the wild flies. A biologically safe higher irradiation dose could result in reduced competitiveness of the VIENNA 8 1260 strain. Mating and remating experiments suggest that Mediterranean fruit fly females exhibit a relative precedence in the use of the sperm: though both sperms are mixed, sperm from the remating is spent first. Results suggest a lower fitness of VIENNA 8 1260 sperm, when compared with sperm from a nonfluorescent bisexual strain, which is consistent with the lower reproductive performance documented for the VIENNA 8 1260 strain.


Resumen en: Inglés |
Resumen en inglés

The Mediterranean fruit fly, Ceratitis capitata (Wiedemann) (Diptera: Tephritidae), is considered one of the most important pests worldwide because of its direct damage to fruit and vegetable production, and restrictions imposed to commercialization of horticultural commodities by countries free of the pest. It was introduced to Brazil in 1901 and to Costa Rica in 1955, from where it spread across the Central American region, reaching Guatemala and Mexico in 1976 and 1977, respectively. In response, the governments of Guatemala, Mexico, and the USA joined efforts to (1) contain further northward spread of the pest, (2) eradicate it from the areas it had invaded in southernMexico, and (3) in the longer termeradicate it from Guatemala and eventually from the rest of Central America. To this effect, cooperative agreements were subscribed between the three countries and also between the USA and Belize. This allowed regional cooperation against the Mediterranean fruit fly and the creation of the Moscamed Programme. The programme was the first area-wide large-scale application of the sterile insect technique (SIT) against this pest. By 1982, the Programme had achieved its first two objectives with the containment of the northward advance of the pest, and its eradication from the areas it had invaded in the states of Chiapas and Oaxaca in southern Mexico. Furthermore, by 1985 the Mediterranean fruit fly had been eradicated from areas in Guatemala located at the border with Mexico. Since then, the programme has had years with significant territorial advances in the eradication of the pest from areas within Guatemala, combined with years when it had setbacks resulting in losses of the territorial gains. Nevertheless, during 4 decades, the programme has effectively served as an effective containment barrier maintaining the Mediterranean fruit fly-free status of Belize, Mexico, and the USA.

It has also protected and increased the Mediterranean fruit fly-free areas in Guatemala. As a result, it has protected the assets of horticultural producers and contributed during this period to the development of multibillion dollar export industries in these countries. This paper provides an historical review of the programme and describes briefly how technological innovations and decision-making tools have contributed to programme efficiency. It also discusses how non-technical and external factors have limited the eradication process and further programme advance within the Central American region.


5.
- Artículo con arbitraje
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Performance of a genetically modified strain of the mediterranean fruit fly (Diptera: Tephritidae) for area-wide integrated pest management with the sterile insect technique
Ramírez Santos, Edwin Mauricio ; Rendón Arana, Pedro Alfonso (coaut.) ; Ruiz Montoya, Lorena (coaut.) (1964-) ; Toledo, Jorge (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Biological and Microbial Control Vol. 110, no. 1 (February 2017), p. 24–34 ISSN: 1938-291X
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Resumen en: Inglés |
Resumen en inglés

The genetically modified strain of Ceratitis capitata (Wiedemann) VIENNA 8 1260 has two morphological markers that exhibit fluorescence in body and sperm. To assess the feasibility of its use in area-wide integrated pest management (AW-IPM) programs using the sterile insect technique, its rearing performance and quality control profile under small, medium, and large scales was evaluated, as well as in field cages. The VIENNA 8 1260 strain had a lower yield than the control strains, VIENNA 8 with D53 inversion (VIENNA 8) and without D53 inversion (VIENNA 8 D53-). At mass-rearing scale, yield gradually increased in three generations without reaching the control strain values. The VIENNA 8 1260 strain was stable in the genetic sexing mechanism (>99.9%) and expression of fluorescence (100%). In field cages, the VIENNA 8 1260 males reduced the mating potential of wild males in the same magnitude as the VIENNA 8, when evaluated in independent cage tests. However, the relative sterility index and the strain male relative performance index of VIENNA 8 1260 males were significantly lower than those of the VIENNA 8. There were no significant differences in longevity of these strains. The potential application of the VIENNA 8 1260 in AW-IPM programs is further discussed.


6.
Tesis - Doctorado
Desempeño de una cepa genéticamente modificada de la mosca del Mediterráneo, Ceratitis capitata (Wied.) para su uso en la TIE / Edwin Mauricio Ramírez Santos
Ramírez Santos, Edwin Mauricio (autor) ; Liedo Fernández, Pablo (director) ; Ruiz Montoya, Lorena (asesora) (1964-) ; Rendón Arana, Pedro Alfonso (asesor) ; Toledo, Jorge (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2016
Clasificación: TE/632.774 / R34
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013581 (Disponible)
Disponibles para prestamo: 1
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Índice | Resumen en: Español |
Resumen en español

La utilización de organismos genéticamente modificados puede contribuir a mejorar la eficiencia de la Técnica del Insecto Estéril (TIE). Recientemente se han desarrollado cepas de la mosca del Mediterráneo, que portan secuencias de ADN que codifican para la expresión de proteínas fluorescentes. Una de estas cepas fluorescentes es la #1260_F-3_m-1 (VIENNA 8 1260), desarrollada a partir de la cepa VIENNA 8, que porta genes que permiten la separación de hembras y machos. La expresión de fluorescencia, producto de la inserción, puede ser aprovechada como marcador para dar mayor eficiencia a la TIE, al identificar en campo de forma precisa el origen de los individuos. Por ser insectos transgénicos el uso de la cepa VIENNA 8 1260 puede tener un costo biológico en su desempeño, el riesgo de transmitir el transgen de fluorescencia a poblaciones silvestres de esta especie y debe cumplir con requisitos de bioseguridad para su liberación en campo. Se realizó una serie de ensayos para determinar la viabilidad del uso de la cepa transgénica en programas que aplican la TIE. Se documentó una mejora generacional, gradual, en el desempeño de la cepa en cría masiva, alta estabilidad y conservación de la fluorescencia del 100%. La competitividad sexual bajo condiciones de jaula de campo fue menor para VIENNA 8 1260 que para la cepa actualmente en uso (VIENNA 8 D53-). Las dosis de irradiación requeridas para la esterilización fueron similares para ambas cepas; sin embargo, para minimizar el riesgo de la trasmisión vertical hacia la población silvestre, se recomiendan dosis mayores a 140 Gy. Se evidenció recópula en hembras, precedencia en el uso de esperma a favor del macho con que recopulan y un mayor desempeño (fitness) del esperma de machos no fluorescentes. No se observó trasmisión horizontal del transgen fluorescente hacia el parasitoide Fopius ceratitivorus durante 16 generaciones.

Índice

Resumen
Capítulo I Introducción
Capítulo II Desempeño de una cepa genéticamente modificada de la mosca de la fruta, Ceratitis capitata (Wied.) para su uso en un programa de manejo integrado de plagas en áreas extensas (MIP-AE) que emplea la técnica del insecto estéril (TIE)
Capítulo III Efectos de la dosis de irradiación sobre la inducción de esterilidad y garantías biológicas de una cepa genéticamente modificada de la mosca mediterránea de la fruta, Ceratitis capitata (Wied.)
Capítulo IV El uso de la PCR como una herramienta de evaluación de la transferencia horizontal de marcadores de ADN entre una cepa genéticamente modificada de la mosca mediterránea de la fruta, Ceratitis capitata (Wied.) y su parasitoide específico Fopius ceratitivorus Wharton (Braconidae)
Capítulo V Conclusiones generales
Literatura citada
Anexos


7.
Tesis - Doctorado
Mejoramiento del desempeño sexual de machos de la cepa de sexado genético de Anastrepha ludens Tapachula-7 / José Luis Quintero Fong
Quintero Fong, José Luis (autor) ; Liedo Fernández, Pablo (director) ; Ruiz Montoya, Lorena (asesora) (1964-) ; Rendón Arana, Pedro Alfonso (asesor) ; Toledo, Jorge (asesor) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2016
Clasificación: TE/632.774 / QU5
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
ECO020013583 (Disponible)
Disponibles para prestamo: 1
PDF
Índice | Resumen en: Español |
Resumen en español

El objetivo del presente estudio fue evaluar la influencia de la selección por apareamiento en el desempeño sexual de machos de la cepa de sexado genético (CSG) de la mosca mexicana de la fruta Anastrepha ludens, llamada Tapachula-7. Dentro de este estudio se plantearon tres objetivos específicos: 1) determinar la factibilidad de mejorar el desempeño sexual de los machos de la CSG mediante un proceso de selección dirigida al apareamiento, 2) caracterizar algunos atributos biológicos de la progenie resultante del proceso de selección dirigida al apareamiento y 3) determinar el grado de heredabilidad de caracteres morfométricos asociados al desempeño de apareamiento de machos de la CSG. El capítulo II describe el trabajo realizado en torno al primer objetivo. La selección por apareamiento mejoró significativamente el desempeño sexual de la progenie. Los resultados del segundo objetivo se encuentran en los capítulos II y III. En estos resultados, se observaron relaciones positivas con la sobrevivencia de larvas, pupas y adultos, con los pesos de larvas y pupas y con la fecundidad. Se observaron relaciones negativas con los tiempos de desarrollo de los estados inmaduros, la cantidad relativa de ciertos compuestos feromonales y la esperanza de vida de los adultos. Finalmente, en el capítulo IV se reportan los resultados del tercer objetivo. El peso de pupa y largo de ala presentaron una heredabilidad media y mayor a la observada en el ancho de cabeza, ancho de tórax y longitud de fémur. Los machos provenientes de padres exitosos presentaron mayor probabilidad de apareamiento que los machos provenientes de padres no exitosos. Estos resultados confirman la viabilidad de mejorar el desempeño sexual de los machos, por medio de la incorporación de métodos de selección en el manejo de la colonia de la cepa Tapachula-7.

Índice

Resumen
Capítulo I
Introducción
Capítulo II
Competitividad sexual y caracterización de atributos biológicos de machos Tapachula-7 seleccionados por apareamiento en presencia de moscas silvestres de Anastrepha ludens
Capítulo III
Sobrevivencia de moscas de la cepa Tapachula-7 seleccionados por apareamiento y moscas silvestres de Anastrepha
Capítulo IV
Heredabilidad de caracteres asociados al desempeño de apareamiento de machos de la cepa Tapachula-7 de Anastrepha ludens
Capítulo V
Conclusiones finales
Literatura citada


8.
- Artículo con arbitraje
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Selection by mating competitiveness improves the performance of Anastrepha ludens males of the genetic sexing strain Tapachula-7
Quintero Fong, José Luis (autor) ; Toledo, Jorge (autor) ; Ruiz Montoya, Lorena (autora) (1964-) ; Rendón Arana, Pedro Alfonso (autor) ; Orozco Dávila, Dina (autora) ; Cruz López, Leopoldo Caridad (autor) ; Liedo Fernández, Pablo (autor) ;
Disponible en línea
Contenido en: Bulletin of Entomological Research Vol. 106, no. 5 (Oct. 2016), p. 624–632 ISSN: 1475-2670
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Resumen en: Inglés |
Resumen en inglés

The sexual performance of Anastrepha ludens males of the Tapachula-7 genetic sexing strain, produced via selection based on mating success, was compared with that of males produced without selection in competition with wild males. Mating competition, development time, survival, mass-rearing quality parameters and pheromone production were compared. The results showed that selection based on mating competitiveness significantly improved the sexual performance of offspring. Development time, survival of larvae, pupae and adults, and weights of larvae and pupae increased with each selection cycle. Differences in the relative quantity of the pheromone compounds (Z)-3-nonenol and anastrephin were observed when comparing the parental males with the F4 and wild males. The implications of this colony management method on the sterile insect technique are discussed.


9.
- Artículo con arbitraje
*En hemeroteca, SIBE-Tapachula
Effects of diet, ginger root oil, and elevation on the mating competitiveness of male mediterranean fruit flies (Diptera: tephritidae) from a mass-reared, genetic sexing straing in Guatemala
Shelly, Todd E. ; Rendón Arana, Pedro Alfonso (coaut.) ; Hernández Ortiz, Emilio (coaut.) ; Salgado García, Sergio (coaut.) ; McInnes, Donald (coaut.) ; Villalobos, Ethel (coaut.) ; Liedo Fernández, Pablo (coaut.) ;
Contenido en: Journal of Economic Entomology Vol. 96, no. 4 (August 2003), p. 1132-1141 ISSN: 0022-0493
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
B3096 (Disponible)
Disponibles para prestamo: 1
Nota: En hemeroteca, SIBE-Tapachula
Resumen en: Inglés |
Resumen en inglés

The release of sterile males is a key component of an areawide program to eradicate the Mediterranean fruit ßy, Ceratitis capitata (Wiedemann), from Guatemala and southern Mexico. The objective of our study was to assess the effects of adult diet, exposure to ginger root oil (Zingiber officinale Roscoe), and elevation on the mating competitiveness of the sterile males used in an areawide program. Sterile males were maintained on a protein-sugar (protein-fed) or a sugar-only (protein-deprived) diet and were exposed (for 4 h 1 d before testing) or not exposed to ginger root oil. In Þeld-cage trials conducted at a high (1,500m) and low(700m) site,wemonitored the inßuence of these treatments on the mating success of sterile males in competition with wild males (reared exclusively on the protein-sugar diet andwithout ginger root oil exposure) forwild females. Elevation and ginger root oil exposure had signiÞcant effects, with sterile males having higher mating success at the low-elevation site and ginger root oil-exposed males having greater success than ginger root oil-deprived males at both sites. Diet did not have a signiÞcant overall effect, and its inßuence varied with elevation (dietary protein seemed to provide an advantage at the high-elevation site but not at the low-elevation site). Possible implications of these Þndings for eradication programs against the Mediterranean fruit ßy are discussed.


10.
- Artículo con arbitraje
*En hemeroteca, SIBE-Tapachula
Consistency in courtship pattern among populations of medfly (Diptera: Tephritidae): comparisons among wild strains and strains mass reared for sit operations
Lux, S. A. ; Munyiri, F. N. (coaut.) ; Vilardi, J. C. (coaut.) ; Liedo Fernández, Pablo (autor) ; Economopoulos, A. (coaut.) ; Hasson, O. (coaut.) ; Quilici, S. (coaut.) ; Gaggl, K. (coaut.) ; Cayol, J. P. (coaut.) ; Rendón Arana, Pedro Alfonso (autor) ;
Contenido en: Florida Entomologist Vol. 85, no. 1 (2002), p. 113-125 ISSN: 0015-4040
Bibliotecas: Tapachula
Cerrar
SIBE Tapachula
24361-30 (Disponible)
Disponibles para prestamo: 1
Nota: En hemeroteca, SIBE-Tapachula
PDF PDF
Resumen en: Español | Inglés |
Resumen en español

El objetivo de este estudio fue de comparar el comportamiento en el cortejo de varias razas de mosca del Mediterráneo salvajes y criadas en masas, de manera de determinar el grado de diversidad en el comportamiento del cortejo entre poblaciones de moscas del Mediterráneo y para poder analizar sus implicaciones para la estrategia de aplicación de la técnica del insecto estéril. Información con respecto al comportamiento del cortejo con la mosca del Mediterráneo fue recolectada de varias localidades alrededor del mundo utilizando un protocolo estándar. Análisis cualitativos y cuantitativos de los materiales de comportamiento recolectados fueron conducidos. No se encontraron mayores diferencias entre las razas, ambas con un repertorio de comportamiento entre machos y hembras, lo cual indica una carencia general de incompatibilidad de comportamiento entre las razas estudiadas. Sin embargo, el análisis reveló varias diferencias cualitativas y cuantitativas en cuanto a detalles en el cortejo entre las localidades. Las hembras de la raza proveniente de Madeira fueron más selectivas que aquellas de otras razas, rechazando el cortejo de los machos con mayor frecuencia a pesar de que los machos de esta raza desplegaron sus actividades de cortejo de la manera más expresiva. Se ha sugerido, por lo tanto que el desarrollo de una raza eficiente para la aplicación a nivel mundial se debe basar en las razas más competitivas tal como la raza de Madeira), y solamente individuos con el patrón más pronunciado de cortejo por parte del macho deben seleccionarse como fundadores.

Resumen en inglés

The objective of the study was to compare courtship behavior of various wild and mass reared medfly strains, in order to document the degree of diversity in courtship behavior among medfly populations and to assess its implications for strategy of application of the Sterile Insect Technique. Recordings of medfly courtship behavior were collected from several locations world-wide using a standard protocol. Qualitative and quantitative analysis of the collected behavioral materials was conducted. No major differences were found among the strains both in male and female behavioral repertoire, which indicates general lack of behavioral incompatibility among the strains studied. However, the analysis revealed several qualitative and quantitative differences in courtship details among locations. The females from Madeira strain were more “choosy” then those from other strains, rejecting male courtship most frequently in spite of the fact that the males from this strain displayed their courtship activities in the most expressed manner. It has been suggested, therefore, that development of an efficient strain for world-wide application shall be based on the most competitive strains (such as Madeira strain), and only individuals with the most pronounced pattern of male courtship should be selected as founders.