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2 resultados encontrados para: AUTOR: Rodríguez Arnaiz, Rosario
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1.
- Artículo con arbitraje
Molecular evidence of hybridization in sympatric populations of the Enantia jethys complex (Lepidoptera: Pieridae)
Jasso Martínez, Jovana M. ; Machkour M'Rabet, Salima (coaut.) ; Vila, Roger (coaut.) ; Rodríguez Arnaiz, Rosario (coaut.) ; Castañeda Sortibrán, América Nitxin (coaut.) ;
Contenido en: PLoS One Vol. 17, no. 5, Art. no. e0197116 (May 2018), p. 1-23 ISSN: 1932-6203
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Resumen en: Inglés |
Resumen en inglés

Hybridization events are frequently demonstrated in natural butterfly populations. One interesting butterfly complex species is the Enantia jethys complex that has been studied for over a century; many debates exist regarding the species composition of this complex. Currently, three species that live sympatrically in the Gulf slope of Mexico (Enantia jethys, E. mazai, and E. albania) are recognized in this complex (based on morphological and molecular studies). Where these species live in sympatry, some cases of interspecific mating have been observed, suggesting hybridization events. Considering this, we employed a multilocus approach (analyses of mitochondrial and nuclear sequences: COI, RpS5, and Wg; and nuclear dominant markers: inter-simple sequence repeat (ISSRs) to study hybridization in sympatric populations from Veracruz, Mexico. Genetic diversity parameters were determined for all molecular markers, and species identification was assessed by different methods such as analyses of molecular variance (AMOVA), clustering, principal coordinate analysis (PCoA), gene flow, and PhiPT parameters. ISSR molecular markers were used for a more profound study of hybridization process. Although species of the Enantia jethys complex have a low dispersal capacity, we observed high genetic diversity, probably reflecting a high density of individuals locally. ISSR markers provided evidence of a contemporary hybridization process, detecting a high number of hybrids (from 17% to 53%) with significant differences in genetic diversity. Furthermore, a directional pattern of hybridization was observed from E. albania to other species. Phylogenetic study through DNA sequencing confirmed the existence of three clades corresponding to the three species previously recognized by morphological and molecular studies.

This study underlines the importance of assessing hybridization in evolutionary studies, by tracing the lineage separation process that leads to the origin of new species. Our research demonstrates that hybridization processes have a high occurrence in natural populations.


2.
- Artículo con arbitraje
Resumen en español

Las regiones de códigos de barras en el ADN han sido utilizadas para la identificación de organismos y delimitación de especies. Nuestra investigación se enfoca en mariposas pertenecientes al complejo Enantia jethys (Lepidoptera: Pieridae) en México, específicamente para resolver el problema taxonómico de cuántas y cuáles especies conforman este grupo. Utilizamos un segmento estándar de aproximadamente 650 pares de bases de la subunidad I del gen mitocondrial citocromo oxidasa (COI). Este estudio es el primero en examinar las relaciones filogenéticas de este complejo de especies utilizando secuencias de ADN. En este estudio fueron empleados tres métodos de inferencia filogenética: parsimonia, máxima verosimilitud, e inferencia bayesiana, también fueron implementados dos métodos de delimitación de especies: generalized mixed Yulecoalescent y Poisson tree process (bPTP). Se obtuvieron 155 secuencias de COI y cuatro grupos monofiléticos consistentes en todos los análisis realizados: Enantia albania (Bates), Enantia jethys (Boisduval), y Enantia mazai Llorente los cuales ya habían sido previamente reportados, además de un cuarto grupo que puede corresponder a una potencial especie críptica en el complejo, la cual debe ser descrita.

Resumen en inglés

DNA barcoding regions have been used for identifying organisms and delimiting species. Our research focused on butterflies belonging to the Enantia jethys species complex (Lepidoptera: Pieridae) in Mexico, specifically to resolve the taxonomic problem of the number of species in the group. We used the standard segment of approximately 650 base pairs of the mitochondrial cytochrome oxidase subunit I (COI) gene. Our study is the first to use DNA sequences to examine phylogenetic relationships of this complex species. Three phylogenetic inference methods used were parsimony, maximum likelihood, and Bayesian inference. Twospecies delimitation methods also were used: generalized mixed Yule-coalescent and Poisson tree process (bPTP). We used all the analyses to obtain 155 COI sequences and a persistent clade with four monophyletic groups: three corresponding to Enantia albania (Bates), Enantia jethys (Boisduval), and Enantia mazai Llorente, and a fourth corresponding to a new potential cryptic species, which must be described.