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2 resultados encontrados para: AUTOR: Scheinvar, Enrique
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Twenty-nine DNA regions of plastid origin have been previously identified in the mitochondrial genome of Cucurbita pepo (pumpkin; Cucurbitaceae). Four of these regions harbor homolog sequences of rbcL, matK, rpl20–rps12 and trnL–trnF, which are widely used as molecular markers for phylogenetic and phylogeographic studies. We extracted the mitochondrial copies of these regions based on the mitochondrial genome of C. pepo and, along with published sequences for these plastome markers from 13 Cucurbita taxa, we performed phylogenetic molecular analyses to identify inter-organellar transfer events in the Cucurbita phylogeny and changes in their nucleotide substitution rates. Phylogenetic reconstruction and tree selection tests suggest that rpl20 and rbcL mitochondrial paralogs arose before Cucurbita diversification whereas the mitochondrial matK and trnL–trnF paralogs emerged most probably later, in the mesophytic Cucurbita clade. Nucleotide substitution rates increased one order of magnitude in all the mitochondrial paralogs compared to their original plastid sequences. Additionally, mitochondrial trnL–trnF sequences obtained by PCR from nine Cucurbita taxa revealed higher nucleotide diversity in the mitochondrial than in the plastid copies, likely related to the higher nucleotide substitution rates in the mitochondrial region and loss of functional constraints in its tRNA genes.

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Genetic variation and relationships among agaves related to the production of Tequila and Mezcal in Jalisco
Trejo, Laura (autora) ; Limones, Verónica (autora) ; Peña, Guadalupe (autora) ; Scheinvar, Enrique (autor) ; Vargas Ponce, Ofelia (autora) ; Zizumbo Villarreal, Daniel (autor) ; Colunga García-Marín, Silvia Patricia (autora) ;
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Contenido en: Industrial Crops and Products Vol. 125 (December 2018), p. 140-149 ISSN: 0926-6690
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Resumen en: Inglés |
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The study of evolutionary history allows us to examine diversification, selection and domestication processes. Mexico belongs to Mesoamerica, one of the world's most important centers of origin and diversification of plants. One of the plants that has sustained its peoples for over 10,000 years is the agave (Agave sp.). Mexico is the center of diversity of the genus, with 75% of the species. Two agave products, tequila and mezcal, are of great economic and biocultural importance for Mexico. The description of genetic diversity and the identification of the wild relatives of the agave species used to produce these emblematic beverages is fundamental information for their production and conservation. Previous studies have proposed wild populations of A. angustifolia in Jalisco as possible wild relatives of blue agave or tequila (Agave tequilana). We use microsatellite (eight loci) to study the genetic diversity and the relationships between wild populations of A. angustifolia and traditional cultivars of the Agave species utilized in the production of tequila and mezcal in Jalisco. The studied taxa present intermediate genetic variation, with the exception of A. tequilana “Azul” which had the same genotype. A Structure analysis indicates that the “Azul” is closely related to A. angustifolia mainly to wild populations from southern Jalisco. Agave rhodacantha and the cultivars of A. tequilana (“Sigüin” and “Chato”) form a group separate from Agave angustifolia y A. tequilana “Azul”.