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19 resultados encontrados para: TEMA: Triatoma dimidiata
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1.
Artículo
*Solicítelo con su bibliotecario/a
Behavioural and electrophysiological responses of Triatoma dimidiata nymphs to conspecific faecal volatiles
Gálvez Marroquín, Zitlalic ; Cruz López, Leopoldo Caridad (coaut.) ; Malo Rivera, Edi Álvaro (coaut.) ; Ramsey Willoquet, Janine M. (coaut.) ; Rojas, Julio C. (coaut.) ;
Contenido en: Medical and Veterinary Entomology Vol. 32, no. 1 (March 2018), p. 102–110 ISSN: 0269-283X
Nota: Solicítelo con su bibliotecario/a
Resumen en: Inglés |
Resumen en inglés

The behavioural and electrophysiological (electroantennography) responses of the first two instars of Triatoma dimidiata (Hemiptera: Reduviidae) Latreille to fresh and dry faecal headspace volatile extracts from fifth instar conspecific nymphs and synthetic compounds were analysed in this study. Recently emerged nymphs (3–5 days) aggregated around filter paper impregnated with dry faeces and around filter paper impregnated with extracts from both fresh and dry faeces. Older first instars (10–15 days) and second instars aggregated around filter paper impregnated with fresh and dry faeces, and their respective headspace extracts. Dry faecal volatile extracts elicited the strongest antennal responses, followed by fresh faecal extracts. Gas chromatography−mass spectrometry analysis of dried faecal headspace volatiles demonstrated the presence of 12 compounds: 2-ethyl-1-hexanol, 1,2,4-trimethylbenzene, n-octadecane, n-nonadecane, n-eicosane, n-heneicosane, n-tricosane, n-pentaeicosane, n-hexaeicosane, n-octaeicosane, nonanal, and 4-methyl quinazoline. In fresh faecal headspace extracts, only nonanal was clearly detected, although there were other trace compounds, including several unidentified sesquiterpenes. Four of the 11 compounds tested individually elicited aggregation behaviour at concentrations of 100 ng/μL and 1 μg/μL. A blend containing these four components also mediated the aggregation of nymphs. These volatiles may be valuable for developing monitoring methods and designing sensitive strategies to detect and measure T. dimidiata infestation.


2.
- Artículo con arbitraje
*Solicítelo con su bibliotecario/a
Electroantennogram responses of the Triatoma dimidiata complex to volatiles produced by its exocrine glands
May Concha, Irving Jesús ; Guerenstein, Pablo G. (coaut.) ; Malo Rivera, Edi Álvaro (coaut.) ; Catalá, Silvia S. (coaut.) ; Rojas, Julio C. (coaut.) ;
Contenido en: Acta Tropica Vol. 185 (September 2018), p. 336-343 ISSN: 0001-706X
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Resumen en: Inglés |
Resumen en inglés

Members of the Triatoma dimidiata complex are vectors of the protozoan parasite Trypanosoma cruzi, the etiologic agent of Chagas disease. Morphological and genetic studies indicate that T. dimidiata complex has three principal haplogroups in Mexico. However, whether there are differences in the olfactory physiology among the haplogroups of this complex and a possible correlation with their antennal phenotype are not yet known. Antennal responses to 13 compounds released from the metasternal and Brindley´s glands, which are involved in the alarm and mating-related behaviours of T. dimidiata were investigated using electroantennography (EAG). Overall, of the 13 compounds tested, seven triggered EAG responses in both sexes of three Mexican haplogroups. The sensitivity of the EAG responses show some relationship with the total number of chemo-sensilla present on the antennae. Antennal sensitivity was different between sexes and haplogroups of the T. dimidiata complex. Discriminant analysis of EAG sensitivity was significant, separating the three haplogroups. Our finding is consistent with morphological and genetic evidence for haplogroups distinction within the complex.


3.
- Artículo con arbitraje
Identificación de volátiles del tlacuache Didelphis marsupialis como atrayente de Triatoma dimidiata (Hemiptera: reduviidae)
Campuzano Granados, Emmanuel Franco (autor) ; Malo Rivera, Edi Álvaro (autor) ;
Contenido en: Entomología Mexicana No. 5 (julio 2018), p. 288−293 ISSN: 2448-475X
PDF
Resumen en: Español | Inglés |
Resumen en español

El objetivo del presente trabajo fue identificar los compuestos liberados por el tlacuache, Didelphis marsupialis como atrayente de Triatoma dimidiata. Por medio de bioensayos realizados en un olfatómetro de vidrio tipo “Y”, se determinó que los volátiles presentes en hembras de tlacuache atrajeron de forma significativa a ninfas de 5to estadio de T. dimidiata; por el contrario, los volátiles de machos no atrajeron a las ninfas. Se Identificaron los compuestos volátiles mediante cromatografía de gases y espectrometría de masas (CG-EM), encontrando la presencia de hidrocarburos, ácidos carboxílico, alcoholes, ésteres y aldehídos. En volátiles de hembras se encontró la presencia de nonanal, cariofileno, curcumeno y de ácido oleico entre otros compuestos que no están presentes en volátiles de machos. Además, se evaluaron de manera individual (p-cresol, nonanal y ácido oleico), y se determinó que las ninfas fueron atraídas solo por el nonanal. Por lo que con este resultados se puede afirmar que este compuesto puede ser un buen candidato a ser evaluado en campo como atrayente de T. dimidiata.

Resumen en inglés

The objective of this work was to identify the compounds released by the opossum, Didelphis marsupialis as attractant of Triatoma dimidiata. T. dimidiata nyphs of 5th were significantly attracted by volatiles released by opossum females in bioassays performance in a “Y" glass-tube olfactometer, on the contrary, volatile released by males opossum did not attract the nymphs. They were identified the volatile compounds by gas chromatography and mass spectrometry (GC-MS), finding the presence of hydrocarbons, carboxylic acids, alcohols, esters and aldehydes. In volatile females the presence of nonanal, caryophyllene, curcumene and oleic acid was found among other compounds that are not present in volatile males. In addition, we tested individually (p-cresol, nonanal and oleic acid), and we found that the nymphs were attracted only by nonanal. Results from this work, suggest that nonanal is a good candidate to be evaluated in the field as an attractant of T. dimidiata.


4.
- Artículo con arbitraje
Antennal phenotype of Mexican haplogroups of the Triatoma dimidiata complex, vectors of Chagas disease
May Concha, Irving Jesús (autor) ; Guerenstein, Pablo G. (autor) ; Ramsey Willoquet, Janine M. (autor) ; Rojas, Julio C. (autor) ; Catalá, Silvia S. (autora) ;
Contenido en: Infection, Genetics and Evolution Vol. 40 (June 2016), p. 73–79 ISSN: 1567-1348
Resumen en: Inglés |
Resumen en inglés

Triatoma dimidiata (Latreille) is a species complex that spans North, Central, and South America and which is a key vector of all known discrete typing units (DTU) of Trypanosoma cruzi, the etiologic agent of Chagas disease. Morphological and genetic studies indicate that T. dimidiata is a species complex with three principal haplogroups (hg) in Mexico. Different markers and traits are still inconclusive regarding if other morphological differentiation may indicate probable behavioral and vectorial divergences within this complex. In this paper we compared the antennae of three Mexican haplogroups (previously verified by molecular markers ND4 and ITS-2) and discussed possible relationships with their capacity to disperse and colonized new habitats. The abundance of each type of sensillum (bristles, basiconics, thick- and thin-walled trichoids) on the antennae of the three haplogroups, were measured under light microscopy and compared using Kruskal–Wallis non-parametric and multivariate non-parametric analyses. Discriminant analyses indicate significant differences among the antennal phenotype of haplogroups either for adults and some nymphal stages, indicating consistency of the character to analyze intraspecific variability within the complex. The present study shows that the adult antennal pedicel of the T. dimidiata complex have abundant chemosensory sensilla, according with good capacity for dispersal and invasion of different habitats also related to their high capacity to adapt to conserved as well as modified habitats.

However, the numerical differences among the haplogroups are suggesting variations in that capacity. The results here presented support the evidence of T. dimidiata as a species complex but show females and males in a different way. Given the close link between the bug's sensory system and its habitat and host-seeking behavior, AP characterization could be useful to complement genetic, neurological and ethological studies of the closely related Dimidiata Complex haplogroups for a better knowledge of their vectorial capacity and a more robust species differentiation.


5.
- Artículo con arbitraje
Resumen en: Inglés |
Resumen en inglés

Landscape interactions of Trypanosoma cruzi (Tc) with Triatoma dimidiata (Td) depend on the presence and relative abundance of mammal hosts. This study analyzed a landscape adjacent to the Calakmul Biosphere Reserve, composed of conserved areas, crop and farming areas, and the human community of Zoh Laguna with reported Chagas disease cases. Sylvatic mammals of the Chiroptera, Rodentia, and Marsupialia orders were captured, and livestock and pets were sampled along with T. dimidiata in all habitats. Infection by T. cruzi was analyzed using mtDNA markers, while lineage and DTU was analyzed using the mini-exon. 303 sylvatic specimens were collected, corresponding to 19 species during the rainy season and 114 specimens of 18 species during dry season. Five bats Artibeus jamaicensis, Artibeus lituratus, Sturnira lilium, Sturnira ludovici, Dermanura phaeotis (Dp) and one rodent Heteromys gaumeri were collected in the three habitats. All but Dp, and including Carollia brevicauda and Myotis keaysi, were infected with predominately TcI in the sylvatic habitat and TcII in the ecotone. Sigmodon hispidus was the rodent with the highest prevalence of infection by T. cruzi I and II in ecotone and domestic habitats. Didelphis viginiana was infected only with TcI in both domestic and sylvatic habitats; the only two genotyped human cases were TcII. Two main clades of T. cruzi, lineages I (DTU Ia) and II (DTU VI), were found to be sympatric (all habitats and seasons) in the Zoh-Laguna landscape, suggesting that no species-specific interactions occur between the parasite and any mammal host, in any habitat. We have also found mixed infections of the two principal T. cruzi clades in individuals across modified habitats, particularly in livestock and pets, and in both haplogroups of T. dimidiata.

Results are contradictory to the dilution hypothesis, although we did find that most resilient species had an important role as T. cruzi hosts. Our study detected some complex trends in parasite transmission related to lineage sorting within the matrix. Intriguingly, TcIa is dominant in terrestrial small wildlife in the sylvatic habitat and is the only parasite DTU found in D. virginiana in the domestic habitat, although its frequency remained constant in sylvatic and ecotone vectors. Bats have a key role in TcVI dispersal from the sylvatic habitat, while dogs, sheep, and humans are drivers of TcVI between domestic and ecotone habitats. Overall, our results allow us to conclude that T. cruzi transmission is dependent on host availability within a highly permeable landscape in Zoh Laguna.


6.
- Artículo con arbitraje
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Resumen en: Inglés |
Resumen en inglés

Vector-borne transmission of Trypanosoma cruzi (VBTTc) is dependent on the concomitant interaction between biological and environmental hazard over the entire landscape, and human vulnerability. Representations and practices of health-disease-care-seeking and territorial appropriation and use were analyzed for VBTTc in a qualitative ethnographic study in the Zoh-Laguna landscape, Campeche, Mexico. In-depth interviews and participatory observation explored representations and practices regarding ethno-ecological knowledge related to vector-transmission, health-disease-care-seeking, and land use processes. The population has a broad knowledge of biting insects, which they believe are all most abundant in the rainy season; the community´s proximity to natural areas is perceived as a barrier to control their abundance. Triatomines are mostly recognized by men, who have detailed knowledge regarding their occurrence and association with mammals in non-domestic fragments, where they report being bitten. Women emphasize the dermal consequences of triatomine bites, but have little knowledge about the disease. Triatomine bites and the chinchoma are “normalized” events which are treated using home remedies, if at all. The neglected condition of Chagas disease in Mexican public health policies, livelihoods which are dependent on primary production, and gender-related knowledge (or lack thereof) are structural circumstances which influence the environment and inhabitants´ living conditions; in turn, these trigger triatomine-human contact. The most important landscape practices producing vulnerability are the activities and mobility within and between landscape fragments causing greater exposure of inhabitants primarily in the dry season.

A landscape approach to understanding vulnerability components of VBTTc from health-disease-care-seeking perspectives and based on territorial appropriation and use, is essential where there is continuous movement of vectors between and within all habitats. An understanding of the structural factors which motivate the population´s perceptions, beliefs, and practices and which create and maintain vulnerability is essential to develop culturally relevant and sustainable community-based VBTTc prevention and control.


7.
- Artículo con arbitraje
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Resumen en: Inglés |
Resumen en inglés

Triatoma dimidiata (Latreille) is a key vector complex of Trypanosoma cruzi, etiologic agent of Chagas disease, as it spans North, Central, and South America. Although morphological and genetic studies clearly indicate existence of at least five clades within the species, there has been no robust or systematic revision, or appropriate nomenclature change for species within the complex. Three of the clades (haplogroups) are distributed in Mexico, and recent evidence attests to dispersal of clades across previously “presumed” monotypic geographic regions. Evidence of niche conservatism among sister species of this complex suggests that geographic dispersal is possible for non-sympatric populations, although no information is available on the behavioural aspects of potential interclade interactions, for instance whether differentiation of chemical signaling or response to these signals could impede communication among the haplogroups. Methods: Volatiles emitted by disturbed bugs, Brindley’s (BGs), and metasternal (MGs) glands were identified using solid-phase micro-extraction (SPME) and gas chromatography coupled mass spectrometry (GC-MS). Volatile compounds emitted by BGs and MGs, and those secreted by disturbed nymphs and adults, of the three Mexican T. dimidiata haplogroups were tested for avoidance behaviour by conspecific nymphs and adults using an olfactometer.

Results: Triatoma dimidiata haplogroups all have three age-related alarm responses: absence of response by early stage nymphs, stage-specific response by 4-5th stage nymphs, and a shared 4-5th nymph and adult response to adult compounds. Disturbed bugs released 15 to 24 compounds depending on the haplogroup, among which were three pyrazines, the first report of these organoleptics in Triatominae. Isobutyric acid from BGs was the most abundant molecule in the response in all haplogroups, in addition to 15 (h1) to 21 (h2 and h3) MG compounds. Avoidance behaviour of disturbed bugs and volatiles emitted by BGs were haplogroup specific, while those from the MG were not. Conclusions: Discriminant and cluster analysis of BG + MG compounds indicate significant separation among the three haplogroups, while alarm response compounds were similar between h2 and h3, both distinct from h1. This latter haplogroup is ancestral phylogenetically to the other two. Our results suggest that alarm responses are a conserved behaviour in the Triatoma dimidiata complex.


8.
Tesis - Maestría
Respuesta comportamental y electrofisiológica de ninfas de Triatoma dimidiata Latreille (Hemiptera: reduviidae) a volátiles de heces de conespecíficos / Zitlalic Gálvez Marroquín
Gálvez Marroquín, Zitlalic ; Cruz López, Leopoldo Caridad (tutor) ; Malo Rivera, Edi Álvaro (asesor) ; Rojas, Julio C. (asesor) ; Ramsey Willoquet, Janine M. (asesora) ;
Tapachula, Chiapas, México : El Colegio de la Frontera Sur , 2014
Clasificación: TE/595.754097275 / G3
Cerrar
SIBE Campeche
ECO040005483 (Disponible)
Disponibles para prestamo: 1
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SIBE Chetumal
ECO030008231 (Disponible)
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ECO010017595 (Disponible)
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ECO020013103 (Disponible)
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ECO050005677 (Disponible)
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PDF
Índice | Resumen en: Español |
Resumen en español

La enfermedad de Chagas es una padecimiento potencialmente mortal causado por el parásito Trypanosoma cruzi Chagas (Protozoa, Mastigophora). Esta enfermedad está distribuida principalmente en América Latina, donde se transmite a los humanos principalmente por las heces de insectos triatominos. A nivel mundial, existen cerca de diez millones de personas infectadas y más de 25 millones están en riesgo de adquirir la enfermedad (OMS, 2012). Triatoma dimidiata Latreille (Hemiptera: Reduviidae) es considerado uno de los vectores más importantes en la propagación de la enfermedad de Chagas y se distribuye desde el norte de América del Sur (Colombia, Venezuela, Ecuador y Perú), en todos los países de América Central y en las zonas costeras de México (Pacífico y Golfo) (Ibarra- Cerdeña et al., 2009). Es la única especie que abarca naturalmente, Norte, Centro y el sur de América, reconociéndose tres genotipos del complejo dimidiata en México (Lehmann et al., 2005; Bargues et al., 2008). Esta especie ha sido encontrada en hábitats domésticos en 14 estados de la Republica (Salazar- Schettino et al., 2005), y se sabe que entre 19 y 34% de los individuos de T. dimidiata que infestan las viviendas se encuentran infectados con T. cruzi (Dumonteil et al., 2004; Monroy et al., 2003; Nakagawa et al., 2005).

Índice

Introducción
Respuesta comportamental y electrofisiológica de ninfas de Triatoma dimidiata a volátiles de heces de conespecíficos (Artículo enviado)
Conclusiones
Literatura citada
Anexos


9.
- Artículo con arbitraje
Volatile compounds emitted by Triatoma dimidiata, a vector of Chagas disease: chemical analysis and behavioural evaluation
May Concha, Irving Jesús ; Rojas, Julio C. (coaut.) ; Cruz López, Leopoldo Caridad (coaut.) ; Millar, Jocelyn G. (coaut.) (1954-) ; Ramsey Willoquet, Janine M. (coaut.) ;
Contenido en: Medical and Veterinary Entomology Vol. 27, no. 2 (Jun 2013), p. 165-174 ISSN: 0269-283X
Resumen en: Inglés |
Resumen en inglés

In this study, we evaluated the responses of Triatoma dimidiata Latreille (Hemiptera: Reduviidae) to volatiles emitted by conspecific females, males, mating pairs and metasternal gland (MG) extracts with a Y-tube olfactometer. The volatile compounds released by mating pairs and MGs of T. dimidiata were identified using solid-phase microextraction and coupled gas chromatography-mass spectrometry (GC-MS). Females were not attracted to volatiles emitted by males or MG extracts; however, they preferred clean air to their own volatiles or those from mating pairs. Males were attracted to volatiles emitted by males, females, mating pairs, pairs in which the male had the MG orifices occluded or MG extracts of both sexes. However, males were not attracted to volatiles emitted by pairs in which the female had the MG orifices occluded. The chemical analyses showed that 14 and 15 compounds were detected in the headspace of mating pairs and MG, respectively. Most of the compounds identified from MG except for isobutyric acid were also detected in the headspace of mating pairs. Both females and males were attracted to octanal and 6-methyl-5-hepten-2-one, and males were attracted to 3,5-dimethyl-2-hexanol. Males but not females were attracted to a seven-compound blend, formulated from compounds identified in attractive MG extracts.


10.
- Libro sin arbitraje
Estrategias de control vectorial de la enfermedad de Chagas: estrategias de control de tritoma dimidiata (Hemiptera, Reduviidae), vector de la enfermedad de Chagas en Yucatán, México / Jhibran Ferral Piña, Laura Huicochea G., Benigno Gomez y Gómez
Ferral Piña, Jhibran ; Huicochea Gómez, Laura (coaut.) ; Gómez y Gómez, Benigno (coaut.) ;
Saarbrücken, Deutschland : Editorial Académica Española , 2012
Clasificación: EE/595.754097265 / F4
Bibliotecas: San Cristóbal
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SIBE San Cristóbal
ECO010017582 (Disponible)
Disponibles para prestamo: 1
Resumen en español

La enfermedad de Chagas es una de las infecciones parasitarias con mayor importancia en América latina. Para el estado de Yucatán, México, el principal vector de esta enfermedad es Triatoma dimidiata, teniendo una tasa de infestación alta en las aéreas rurales y media en las urbanas. Debido a las dificultades asociadas con la terapéutica actual del tratamiento y el elusivo desarrollo de una vacuna eficaz, el control se sigue basándose en el control de vectores. Se presentan los resultados de una investigación con enfoque participativo sobre la evaluación de tres estrategias de control del vector T. dimidiata (Barrera física, control químico, patio limpio y testigo), factores de riesgo de reinfestación del vector en las viviendas tratadas, aceptación y satisfacción de las estrategias utilizadas para el control del vector y el conocimiento popular de los pobladores sobre el vector así como sus prácticas de control en cuatro comunidades del estado de Yucatán.

Índice

Introducción
Antecedentes
La enfermedad de Chagas
El vector: Triatoma dimidiata (Latreille, 1811) (Hemiptera: Reduviidae)
Estrategias de control vectorial
Los métodos de control aplicados a Triatoma dimidiata
Conocimiento popular de triatominos vectores de la enfermedad de Chagas
Objetivos
General
Específicos
Metodología
Zona de estudio y diseño del proyecto
Colecta del vector con participación comunitaria
Cuantificación de la colecta de T. dimidiata
Aplicación de las estrategias de control
Valoración de las estrategias de control
Factores que permiten la reinfestación de T. dimidiata en las comunidades
Evaluación de la Aceptación - Satisfacción de los métodos en las comunidades de estudio
Conocimiento popular de las comunidades estudiadas sobre T. dimidiata y su control
Resultados
Valoración de las estrategias de control
Factores asociados a la reinfestación de T. dimidiata en las comunidades
Aceptación y satisfacción de las estrategias de control
Conocimiento popular y prácticas de control de T. dimidiata
Discusión
Valoración de las estrategias de control
Conclusiones
Recomendaciones
Agradecimientos
Literatura Citada
Anexo I
Anexo II
Anexo III