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5 resultados encontrados para: AUTOR: Zamilpa, Alejandro
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This study was aimed at evaluating thein vitroeffect of the edible mushroom (EM) Pleurotus eryngiiagainst the eggs and larvae (L3) of Haemonchus contortus. The evaluation included acetone (AE) and hydroalcoholic (HA) extracts of the following strains: ECS-1138, ECS-1156, ECS-1255, ECS-1258, ECS-1261, ECS-1282, and ECS-1292. The HA extract of the ECS-1255 strain showed thehighest effect on mortality rates of L3 (18.83%) at 20 μg/mL. After subjecting this HA extract to a normal phase chromatography column, five fractions were obtained; fraction F5 (100% MeOH) was the most effective against eggs, with hatching inhibition percentages of 88.77 and 91.87% at 20 and 40 mg/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) subjected this fraction to an acetylation reaction to determine the content of the secondary metabolites. The GC-MSanalysis showed that the F5 fraction was composed of trehalose CAS: 6138-23-4, polyols (L-iditol CAS: 488-45-9, galactitol CAS:608-66-2, D-mannitol CAS: 69-65-8, D-glucitol CAS: 50-70-4, and myoinositol CAS: 87-89-8), adipic acid CAS: 124-04-9, stearic acid CAS: 57-11-4, squalene CAS: 111-02-4, and β-sitosterol CAS: 83-46-5.

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In vitro nematocidal activity of commercial fatty acids and β-sitosterol against Haemonchus contortus
Pineda Alegría, Jesús Antonio (autor) ; Sánchez, José E. (autor) ; González Cortázar, Manáses (autor) ; Von Son de Fernex, Elke (autora) ; González Garduño, Roberto (autor) ; Mendoza de Gives, Pedro (autor) ; Zamilpa, Alejandro (autor) ; Aguilar Marcelino, Liliana (autora) ;
Contenido en: Journal of Helminthology Vol. 94, e135 (2020), p. 1-4 ISSN: 1475-2697
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Haemonchus contortusis a haematophagous gastrointestinal nematode (GIN) that causes severe anaemia and even death in small ruminants, negatively impacting the economic viability of farms. Traditionally, this parasite has been controlled with chemical compounds; however, inadequate use of these types of products has favoured the emergence of anthelmintic resistance. Therefore, it is necessary to search for alternatives for GIN control. Previous studies have reported the anthelmintic activity of edible mushroom extracts against H. contortus. A recent study reported that a fraction constituted of different fatty acids and β-sitosterol isolated from the basidiomata of the edible mushroom Pleurotus djamor ECS-123 has ovicidal and larvicidal activity against H. contortus. Thus, this study aimed to assess the anthelmintic activity of the pure molecules: pentadecanoic acid, palmitic acid, β-sitosterol, stearic acid and linoleic acid. For this purpose, an egg-hatching inhibition test was carried out in which the compounds were evaluated individually and in combination at a final concentration of 20 mg mL−¹. Furthermore, larval mortality was assessed using a combination of the five commercial compounds previously mentioned at different concentrations (1.25–20 mg mL−¹). Palmitic acid and stearic acid, in some combinations, inhibited H. contortus egg hatching by 100%. On the other hand, in the larval mortality test, the combination of the five compounds showed dose-dependent behaviour, and 100% mortality was obtained 24 h post-incubation. Pure molecules and their combinations have anthelmintic-like activity against the eggs and larvae of H. contortus.

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Gastrointestinal parasitic nematodes cause important economic harm to animal health, seriously affecting the livestock industry. Worldwide, economic losses from nematodiasis is reported at more than USD 10 billion a year in anthelmintic treatments alone in 2013. In Mexico, the livestock industry has reported losses of about $8.902 million Mexican pesos in the zootechnical potential of ruminants that interfere in the profitability of livestock farms. So far, these diseases have been controlled by use of anthelmintic products (AH). In addition, improper use of AH contributes to the imbalance of the environment, as well as an ecotoxicological risk to soil, plants, aquifers and beneficial organisms. In this context, the search for alternative and sustainable complementary methods that reduce the need for use of synthetic products of chemical origin, for example natural derivatives of edible fungi with a nematicidal effect, is evident. The edible fungi Pleurotus spp. possesses nutraceutical and therapeutic properties including their use as anti-parasitic. In the present investigation, in vitro effects of compounds derived from Pleurotus spp. were assessed against various stages of the parasitic nematode Haemonchus contortus. Pleurotus ostreatus had higher nematicidal activity than P. eryngii. The "F" fraction of hydroalcoholic extract of P. ostreatus mycelium showed the highest nematicidal activity (25.29%) with respect to other fractions.

FpMeOH and FMeOH fractions had the highest L4 mortality rate of 84.8 and 100, respectively at 24 and 72 h. The spent mushroom substrates (SMS) of P. djamor had biological activity against H. contortus in its egg stages and L3. Furthermore, such activity is possibly influenced by the composition and/or the type of substrate. EHA-SA11 was lethal against L3 exsheathed with a 45% mortality at 20 mg/ml at 72 h postexposure, but did not affect nematode eggs. In contrast, EHA-SA13 was the most effective against parasite eggs with 100% inhibition starting at 2.5 mg/ml that was determined at CL90= 625 μg/ml. The use of a "nutraceutical" food based on SMS of edible mushroom culture represents a potential method of prevention of nematode infections.


- Libro con arbitraje
Updates on tropical mushrooms. Basic and applied research / José E. Sánchez, Gerardo Mata and Daniel J. Royse, editors
Sánchez, José E. (ed.) ; Mata, Gerardo (coed.) ; Royse, Daniel Joseph (coed.) ;
San Cristóbal de Las Casas, Chiapas, México : El Colegio de la Frontera Sur , 2018
Clasificación: EE/635.8 / U6
Bibliotecas: Campeche , San Cristóbal , Tapachula
SIBE Campeche
ECO040007032 (Disponible)
Disponibles para prestamo: 1
SIBE San Cristóbal
ECO010019577 (Disponible)
Disponibles para prestamo: 1
SIBE Tapachula
ECO020013831 (Disponible)
Disponibles para prestamo: 1

1 Importance and Potential of Tropical Mushrooms
Biodiversity and Taxonomy
2 tropical species of Agaricus
3 an overview of tropical and subtropical species of Agaricus in México
4 development of the straw mushroom sector in China
5 Enhanced Production of the Medicinal Mushroom Agaricus Subrufescens Peck
6 Artificial Cultivation of the Medicinal Mushroom Sparassis latifolia
7 Effect of Soak Time After Harvest on Nutritional Quality of Tremella Fuciformis Dry Product
8 Fruit Body Production of Schizophyllum Commune
9 Basidiome production of guatemalan strains of Lepista nuda
10 Shiitake cultivation on straw: an alternative for Subtropical regions
Biotechnological Applications
11 Auricularia spp.: edible mushroom with broad biotechnological properties
12 Antioxidant Activity and Chemical Composition of Grifola Frondosa
13 Immunomodulating and antitumor properties of Pleurotus sp. in Cuba
14 Cultivation Biotechnology for Volvariella spp. in Mexico: Advances, Challenges and Perspectives
15 A Pleurotus spp. hydroalcoholic fraction possess a potent in vitro ovicidal activity against the sheep parasitic nematode Haemonchus contortus
1 Mushrooms mentioned in this book
2 Tropical species of Agaricus